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Buffer Preparation

Author(s): John Jungck1, Annelise Myers1, Stephanie Gage1

Beloit College

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Summary:
This Excel workbook calculates the necessary components to create a buffer solution using the Henderson-Hasselbalch equation.

Licensed under CC Attribution-NonCommercial-ShareAlike 4.0 International according to these terms

Version 1.0 - published on 15 Aug 2024 doi:10.25334/PYSP-0K38 - cite this

Keywords

  1. buffer solution
  2. acid
  3. base

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  2. Subdiscipline
  3. Audience Level
  4. Software Used
Contents:
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Overview

This workbook calculates the necessary components to create a buffer solution. The workbook will allow the user to create a buffer in two ways. A user can select an acid and its conjugate base or select an acid or base and combine it with a strong base or strong acid. Users will input the desired pH, concentration and volume of the solution and the workbook will output a recipe for a buffer solution using the Henderson-Hasselbalch equation.

Popular Text Citations

Moore, J.W., C.L. Stanitski and P.C. Jurs, (2002). Chemistry The Molecular Science. Harcourt College Publishers, Orlando

Jack, R. Cecil. (1995) Basic Biochemical Laboratory Procedures and Computing. Oxford University Press, New York

Harris, D.C., (1987). Quantitative Chemical Analysis. W.H. Freeman and Company, New York. Second Edition.

Research Articles

Good, N.E.; Wingset, G.D.; Winter, W.; Connolly, T.N.; Izawa, S.; Singh, R.M.M. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry, 5:467-477.

Po, H.N.; Senozan, H.M. (2001) The Henderson-Hasselbalch Equation: Its History and Limitations. Journal of Chemical Education, 78: 1499-1503.

Tutorial & Background Materials

Martin Chaplin. Biological Chemistry (Snapshot: 2012-03-31)

"Chemistry and Background" (2003) . ChemLab Darthmouth College. Trustees of Darthmouth College. (Snapshot: 2016-05-07)

Citation

Researchers should cite this work as follows:

Fundamental Mathematical Concepts

Fundamental Mathematical Concepts
Henderson-Hasselbalch

Developed By

Developed by
L.J. Henderson and K.A. Hasselbalch

Primary Reference

Hasselbalch, K. A. 1916. Biochemische. Zeitschrift 78: 112-144.

Lawrence J. Henderson. 1908. Concerning the relationship between the strength of acids and their capacity to preserve neutrality. Am. J. Physiol. 21:173-179.