ESTEEM

Overview

In this simulation, equations used in enzyme kinetics (Michaelis-Menten, Eadie-Hofstee, Dixon, and Lineweaver-Burk) are modeled under various conditions. The equations are modeled in situations with measurement errors in substrate concentration and/or reaction velocity to allow students to observe how these affect output. Students then manipulate values to find the best model of an enzyme reaction. 

Popular Text Citations

Cleland, W. W. (1979). "Statistical analysis of enzyme kinetic data." Methods in Enzymology ,QJ.:103-138.

Fantini, Bernardino. (1988). Jaques Monod: Pour une ethique de la connaissance. (Historie des sciences se- ries). La Decouverte: Paris, France.

Jack, R. Cecil. (1995). Basic Biochemical Laboratory Pro- cedures and Computing.

Fruton, Joseph S. (1972). Molecules and Life: Historical Essays on the Interplay of Chemistry and Biology. Wiley-Interscience: New York, New York.

Cornish-Bowden, (1995) Fundamentals of Enzyme Kinetics, 2nd Edition. Portland Press: London, United Kingdom.

Research Articles

Dowd, J. E. and D. S. Riggs. (1965)." A comparison of estimates of Michaelis-Menten kinetic constants from various linear transformations." Journal of Biological Chemistry 2..4.Q:863.

Nimmo, I. A. and G. L. Atkins. (1979). "The statistical analysis of non-normal (real?) data." Trends in the Biochemical Sciences ,4:236-238.

Education Research & Pedagogical Materials

Smith, D. B. (1979). "The Menten of the Michaelis- Menten equation." TIBS .4: N150.

Tutorial & Background Materials

Fitting Michaelis-Menten parameters to nutrient up- take data (Mineral Nutrition of Plants, SoilScience/Botany /Horticulture 626, Phillip Barak and Edgar Spalding, Instructors, University of Wisconsin-Madison) (based on: A method for characterizing the relation between nutrient con- centration and flux into roots of intact plants. N. Classen and S.A. Barber. (1974.) Plant Physiol 54:564-568.

Kinetics of Enzymatic Reactions: The Michaelis-Menten Equation

Citation