Support

Support Options

  • Knowledge Base

    Find information on common questions and issues.

  • Support Messages

    Check on the status of your correspondences with members of the QUBES team.

Contact Us

About you
About the problem
Resource Image

GEA Introductory lesson on BLAST

Author(s): Justin DiAngelo1, Alexis Nagengast2, Wilson Leung3, Douglas L Chalker3

1. Penn State Berks 2. Widener University 3. Washington University in St. Louis

419 total view(s), 349 download(s)

0 comment(s) (Post a comment)

Summary:
The "Introduction to BLAST using Human Leptin" exercise aims to introduce students to the use of the Basic Local Alignment Search Tool (BLAST) to identify related sequences and compare similarity between them.

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

Version 1.0 - published on 25 Jul 2020 doi:10.25334/WQ1J-PW32 - cite this

Description

This lesson aims to introduce students to the use of the Basic Local Alignment Search Tool (BLAST) to identify related sequences and compare similarity between them. This exercise uses the example of the human leptin gene. It begins with introducing students to the use of NCBI databases to explore diseases and associated genes including the recovery of a desired sequence that is then used to demonstrate the use of BLAST to find related sequences. Formative assessments are imbedded within the lesson to lead the student to consider their search results. A summative assessment is used to help the students reflect on the uses of databases and BLAST to explore functional relationships of genes and genomes. Intended to be used as a stand-alone module to teach fundamental analysis skills with BLAST. Could be used as an in-classroom lesson or as online curriculum to prepare students for individual BLAST searches performed with students in class. Results for the Leptin example BLAST search have been cached to allow for rapid recovery of results when implementing with larger enrollment courses.

Cite this work

Researchers should cite this work as follows:

Comments

There are no comments on this resource.