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Module 4: Removal of Introns from pre-mRNA by Splicing

Author(s): Meg Laakso1, Anne Rosenwald2

1. Eastern University 2. Georgetown University

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Summary:
In this module, students will learn to identify splice donor and acceptor sites that are best supported by RNA-Seq data, and use the canonical splice donor and splice acceptor sequences to identify intron-exon boundaries.

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

Version 1.0 - published on 25 Jul 2020 doi:10.25334/030X-Q219 - cite this

Description

All RNAs in the cell are collectively known as the "transcriptome" as almost all RNA is produced by transcription from a DNA template. (In some cases, RNA is made from an RNA template.) The transcriptome includes messenger RNAs, ribosomal RNAs, transfer RNAs, and other RNAs that have specialized functions in the cell. Here, we will investigate how mRNA specifically is modified. Students will learn to identify splice donor and acceptor sites that are best supported by RNA-Seq and TopHat splice junction predictions and use the canonical splice donor and splice acceptor sequences to identify intron-exon boundaries.

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