Module 4: Removal of Introns from pre-mRNA by Splicing
Author(s): Meg Laakso1, Anne Rosenwald2
1. Eastern University 2. Georgetown University
1255 total view(s), 3656 download(s)
Module_4_Cover_Page.docx(DOCX | 32 KB)
Module_4_Cover_Page.pdf(PDF | 73 KB)- Module_4_Splicing.docx(DOCX | 9 MB)
- Module_4_Splicing.pdf(PDF | 8 MB)
- Module_4_Splicing_Answer_Sheet.docx(DOCX | 44 KB)
- Module_4_Splicing_Answer_Sheet.pdf(PDF | 35 KB)
- Module_4_Splicing_Homework.docx(DOCX | 2 MB)
- Module_4_Splicing_Homework.pdf(PDF | 1 MB)
- Module 4: Removal of Introns from pre-mRNA by Splicing (Read The Docs Version)
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Description
All RNAs in the cell are collectively known as the "transcriptome" as almost all RNA is produced by transcription from a DNA template. (In some cases, RNA is made from an RNA template.) The transcriptome includes messenger RNAs, ribosomal RNAs, transfer RNAs, and other RNAs that have specialized functions in the cell. Here, we will investigate how mRNA specifically is modified. Students will learn to identify splice donor and acceptor sites that are best supported by RNA-Seq and TopHat splice junction predictions and use the canonical splice donor and splice acceptor sequences to identify intron-exon boundaries.
Cite this work
Researchers should cite this work as follows:
- Laakso, M., Rosenwald, A. (2020). Module 4: Removal of Introns from pre-mRNA by Splicing. Genomics Education Alliance, QUBES Educational Resources. doi:10.25334/030X-Q219