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Summary:
This lab manual describes a multi-week lab project for the design, assembly from oliognucleotides, cloning, and sequencing of the RFP gene. This resource was developed as part of NSF Award #1827204, RCN-UBE: The Build-a-Genome Network.
Contents:
Build-a-Genome Lab Manual for RFP Synthesis.docx(DOCX | 462 KB)
Build-a-Genome Lab Manual for RFP Synthesis.pdf(PDF | 627 KB)- License terms
Description
In the Build-a-Genome course, these exercises are frequently used in the first 3-4 weeks as a "boot camp" to introduce students to the techniques and concepts of synthetic genome assembly.
Cite this work
Researchers should cite this work as follows:
- Scheifele, L. (2020). Build-a-Genome Lab Manual for RFP Synthesis. Build A Genome Network, QUBES Educational Resources. doi:10.25334/AN0R-BS96