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Build-a-Genome Lab Manual for RFP Synthesis

Author(s): Lisa Scheifele

Loyola University Maryland

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This lab manual describes a multi-week lab project for the design, assembly from oliognucleotides, cloning, and sequencing of the RFP gene. This resource was developed as part of NSF Award #1827204, RCN-UBE: The Build-a-Genome Network.

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

Version 1.0 - published on 28 Jul 2020 doi:10.25334/AN0R-BS96 - cite this


In the Build-a-Genome course, these exercises are frequently used in the first 3-4 weeks as a "boot camp" to introduce students to the techniques and concepts of synthetic genome assembly.

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