Functional and structural analysis of Amylase activity
Author(s): Ishrat Rahman1, Aubrey Allen Smith1, Evdokia Kastanos2
1. Montgomery College 2. Montgomery College/Rockville
873 total view(s), 2800 download(s)
Summary:
This exercise was developed to allow students to apply the scientific method and use their knowledge of enzymes to investigate the relationship between enzyme activity and structure. Students design experiments to study the effect of environmental…
Contents:
Amylase Activity Lab QUBES.v4.docx(DOCX | 595 KB)
Amylase Activity Lab QUBES.v4.pdf(PDF | 636 KB)- APPENDIX B C.docx(DOCX | 24 KB)
- APPENDIX B C.pdf(PDF | 242 KB)
- License terms
Description
Upon completion of this exercise, the student should be able to:
- design experiment(s) with appropriate controls to test their hypothesis.
- make a standard curve for estimation of maltose concentration.
- set up enzyme-catalyzed (amylase) hydrolysis of starch into maltose.
- explain the relationship between amount of maltose produced and the activity of amylase.
- obtain amino acid sequences of amylase from the NCBI online database.
- use bioinformatics tools to analyze primary structures, compare amino acid sequences and generate a 3D structure of each enzyme.
Cite this work
Researchers should cite this work as follows:
- Rahman, I., Smith, A. A., Kastanos, E. (2020). Functional and structural analysis of Amylase activity. QUBES Educational Resources. doi:10.25334/VKGJ-VT46