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Analysis of Amylase Activity

By Allison Carter Burlyn1, Christina Fieber1, Melanie Lenahan2

1. Horry-Georgetown Technical College 2. Raritan Valley Community College

This lab is an exploration of enzyme function and the effects of environmental conditions on the activity of the enzyme amylase. The lab utilizes analytical and graphing skills to assess enzyme activity.

Listed in Teaching Materials | resource by group Catalytic Graphers

Version 1.0 - published on 24 Nov 2020 doi:10.25334/CW0W-Y495 - cite this

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

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Description

Upon completion of this lab activity, the student will be able to:

  • Describe the components of an enzymatic reaction.
  • Communicate the function of an enzyme’s active site and its relationship to the substrate.
  • Describe the relationship between an enzyme’s structure and its function.
  • Explain the relationship between maltose produced and enzymatic (amylase) activity.
  • Analyze the effect of environmental factors on enzymatic activity.
  • Define absorbance and transmittance and utilize data obtained with a spectrophotometer.
  • Describe the relationship between absorbance and concentration.
  • Construct a standard curve using known amounts of maltose.
  • Utilize a standard curve to determine enzyme activity of the experimental amylase.

Adapted from Amylase Activity Lab, Montgomery College, Rockville, MD (doi:10.25334/VKGJ-VT46).

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