Measuring Size and Area of Digitized Specimens using ImageJ
Author(s): Elissa Suphapun Sorojsrisom1, Janice L. Krumm2, Paula A Trillo3
1. Columbia University 2. Widener University 3. Gettysburg College
1403 total view(s), 2754 download(s)
CURE_CORE_ImageJ_InstructorGuide.pdf(PDF | 255 KB)- CURE_CORE_ImageJ_InstallingImageJ.pdf(PDF | 244 KB)
- CURE_CORE_ImageJ_WingLengthWidth.pdf(PDF | 771 KB)
- CURE_CORE_ImageJ_WingSpotArea.pdf(PDF | 908 KB)
- CURE_CORE_ImageJ_WingApexMelanization.pdf(PDF | 697 KB)
CURE_CORE_ImageJ_ImageAnalysisAssignment_Spreadsheet.xlsx(XLSX | 11 KB)- CURE_CORE_ImageJ_ImageAnalysisAssignment_Key.xlsx (Instructors only)(XLSX | 12 KB)
Fixed Angle Line Tool.ijm(IJM | 898 B )
CURE_CORE_ImageJ_InstallingImageJ.docx(DOCX | 1 MB)- CURE_CORE_ImageJ_WingLengthWidth.docx(DOCX | 11 MB)
- CURE_CORE_ImageJ_WingSpotArea.docx(DOCX | 7 MB)
- CURE_CORE_ImageJ_WingApexMelanization.docx(DOCX | 7 MB)
- License terms
Description
INTRODUCTION
In this activity, students will learn to measure morphological characters using the open access program ImageJ. Students will practice using essential ImageJ tools as they analyze wing size, wing spot areas, and wing apex melanization in images of Pieris rapae, the cabbage white butterfly. While a single species is used in this example, the image analysis skills learned are transferable to other organisms and other morphological measurements. This resource can be used as part of the implementation of a BCEENET CURE, or as a standalone guide for introducing students to specimen analysis using ImageJ.
Intended Audience: Undergraduate, all levels. Suitable for in-person, hybrid, or online courses in biology or environmental science.
Learning Time: Flexible, 2-3 hours
Required Resources: Access to computers and the open access program ImageJ
STUDENT LEARNING OBJECTIVES
- Interpret images of Pieris rapae to determine suitability for image analysis
- Practice using several measurement tools (line, fixed angle line, wand, versatile wand) in ImageJ
- Gather data on wing size, spot area, and apex melanization of P. rapae specimens
CURRICULUM MATERIALS PROVIDED
- Instructor Guide
- Installing ImageJ and Tools
- Fixed Angle Line Tool macro file (.ijm)
- Measuring Wing Size using ImageJ
- Measuring Wing Spot Area using ImageJ
- Measuring Apex Melanization using ImageJ
- Image Analysis Assignment Spreadsheet
- Image Analysis Assignment Key
Cite this work
Researchers should cite this work as follows:
- Sorojsrisom, E. S., Krumm, J. L., Trillo, P. A. (2022). Measuring Size and Area of Digitized Specimens using ImageJ. BCEENET- Biological Collections in Ecology & Evolution Network, QUBES Educational Resources. doi:10.25334/P8QG-AB71