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Yeast Scramble Lab Manual v3

Author(s): Mark Jones, Nick Matinyan1, John Mirarchi2, Lisa Scheifele2

1. Johns Hopkins School of Medicine 2. Loyola University Maryland

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Summary:
This laboratory manual includes protocols for SCRaMbling of yeast cells as well as phenotypic characterization. Appropriate student exercises are also included. This resource was developed as part of NSF Award #1827204, RCN-UBE: The Build-a-Genome…

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This laboratory manual includes protocols for SCRaMbling of yeast cells as well as phenotypic characterization. Appropriate student exercises are also included. This resource was developed as part of NSF Award #1827204, RCN-UBE: The Build-a-Genome Network

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

Version 2.0 - published on 22 Aug 2022 doi:10.25334/E8TR-EE48 - cite this

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Description

The Synthetic Yeast Project Sc2.0 developed synthetic yeast chromosomes with LoxPsym sites scattered throughout the chromosomes. By inducing the expression of Cre recombinase, new chromosome configurations can be generated in yeast cells. This population can then be screened for enhanced or altered phenotypes.

Notes

This second version includes more details in the protocols to clarify pre-lab preparation, supply lists and media recipes, and protocols for analysis of next-generation (Illumina) sequencing data.

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