- Open google colab - https://colab.research.google.com/
- Login with your gmail account to enable google drive access. We will use google drive to store and run our script.
- Create a new notebook
- Create the following two folders
- output
- tmp
- Create an input file (input.txt) with a list of accession numbers
- Copy paste the "blast.py" file to colab and save your notebook
- Run blast
| Query Cover % | E_Value | Accession Id | Subject Name |
|---|---|---|---|
| 99.72 | 0 | WP_136022800 | Streptococcus pyogenes |
| 99.72 | 0 | WP_111679348 | Streptococcus dysgalactiae |
| 99.72 | 0 | WP_138083809 | Streptococcus dysgalactiae |
| 99.72 | 0 | WP_136284882 | Streptococcus pyogenes |
| 99.72 | 0 | WP_032460164 | Streptococcus pyogenes |
| 99.72 | 0 | WP_110410812 | Streptococcus pyogenes |
| 99.72 | 0 | WP_136263979 | Streptococcus pyogenes |
| 99.72 | 0 | WP_136298220 | Streptococcus pyogenes |
| 96.36 | 0 | WP_136037644 | Streptococcus pyogenes |
| 96.36 | 0 | WP_136132953 | Streptococcus pyogenes |
| TOP HIT = WP_136022800 Streptococcus pyogenes |
| Query Cover % | E_Value | Accession Id | Subject Name |
|---|---|---|---|
| 99.72 | 0 | WP_136022800 | Streptococcus pyogenes |
| 99.72 | 0 | QBX23717 | Streptococcus phage Javan146 |
| 99.72 | 0 | WP_136112598 | Streptococcus pyogenes |
| 95.84 | 0 | WP_002990034 | Streptococcus pyogenes |
| 95.84 | 0 | WP_010922218 | Streptococcus pyogenes |
| 99.72 | 0 | WP_014635509 | Streptococcus pyogenes |
| 95.84 | 0 | WP_136040266 | Streptococcus pyogenes |
| 95.84 | 0 | WP_020833526 | Streptococcus pyogenes |
| 95.84 | 0 | WP_046177708 | Streptococcus dysgalactiae |
| 99.72 | 0 | WP_136111941 | Streptococcus pyogenes |
| TOP HIT = WP_136022800 Streptococcus pyogenes |