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The Ames Test

Author(s): Nathan Goodson-Gregg, Elizabeth A. De Stasio

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Description

This published laboratory introduces students to the Ames test, an assay used to determine the mutagenic properties of chemical compounds, using a distinctly quantitative approach. Students must develop their own hypotheses, use proper positive and negative controls, perform their own serial dilutions, use sequencing and bioinformatics to assess their results, and finally, write up their findings in a formal laboratory report.

Citation:

Goodson-Gregg, N. and De Stasio, E. A. (2009) Reinventing the Ames Test as a Quantitative Lab That Connects Classical and Molecular Genetics. Genetics 181: 23-21. DOI: 10.1534/genetics.108.095588

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