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#165, v1.0 Published:
#599, v1.0.0 Published:

Title

Old VersionNew Version
1Using DNA Subway to Analyze Sequence Relationships 1Intro to DNA Subway Blue Line

Authors

Old VersionNew Version
  1Oliver Hyman (The Department of Biology, James Madison University; The Center for Genome & Metagenome Studies, James Madison University ) 
  2Andrea Pesce (James Madison University) 
  3Emily Lescak () 
  4William Tapprich (University of Nebraska-Omaha) 
  5Jason Williams (DNA Learning Center) 
  6Elizabeth Ryder (Worcester Polytechnic Institute) 
  7Ray A Enke (The Department of Biology, James Madison University; The Center for Genome & Metagenome Studies, James Madison University ) 
  8Sam S Donovan (University of Pittsburgh) 
1Hayley Orndorf (University of Pittsburgh) 9Hayley Orndorf (University of Pittsburgh) 
2Jason Williams (DNA Learning Center) 10Hayley Orndorf ()
   11Elizabeth Doyle (James Madison University)

Description

Old VersionNew Version
1<p>These are instructions for a 120-minute bioinformatics lab in which students will learn how to use the DNA Subway Blue Line workflow to determine the species of an organism using a DNA sequence.</p>  1<p>Materials include:</p>
2  2  
3<p>This activity is appropriate for a broad audience with an introductory level experience with bioinformatics. The activity is designed for a computer lab setting with a class size between 10 and 50 students.&nbsp;</p> 3<ol>
   4   <li>A lab activity (with answers included) introducing DNA Barcoding, DNA Subway Blue Line and a short Blue Line activity (HYMAN et al. 2016 DNA Subway Blue Line Lab KEY.docx)</li>
   5   <li>A electronic lab notebook to help students record the data they generate in Blue Line (HYMAN et al. DNA subway lab notebook entry.docx)</li>
   6   <li>Two slide decks that help guide students through DNA Subway Blue Line. The first slide deck is a self-guided tour for students to use on their own (HYMAN et al. DNA Subway_Independent Blue Line Practice.pptx). The second slide deck is designed for an instructor led tour in class (HYMAN et al. DNA Subway_Instructor led Blue Line Practice.pptx).&nbsp;</li>
   7   <li>A slide deck designed to help instructors deal with common issues that can arise during blue line analyses (HYMAN et al. worked example slides for instructors.pptx).</li>
   8   <li>Two &quot;unknown&quot; Sanger sequencing reads (.ab1 files) for the Blue Line activity.</li>
   9</ol>

Attachments

1 file — ./2 Hour Lesson Plan on DNA Barcoding and Blue Line.docx 1 file — ./Hyman Adaptation/1-plant-pos-fall15F_PREMIX_J43474_3.ab1
2 file — ./barcoding_intro_slides.pptx 2 file — ./Hyman Adaptation/1-plant-pos-fall15R_PREMIX_J43475_4.ab1
3 file — ./Hyman Adaptation/HYMAN et al. 2016 DNA Subway Blue Line Lab KEY.docx
4 file — ./Hyman Adaptation/HYMAN et al. DNA subway lab notebook entry.docx
5 file — ./Hyman Adaptation/HYMAN et al. DNA Subway_ Independent Blue Line Practice.pptx
6 file — ./Hyman Adaptation/HYMAN et al. DNA Subway_ Instructor led Blue Line Practice.pptx
7 file — ./Hyman Adaptation/HYMAN et al. worked example slides for instructors.pptx
8 file — publication_647_677/dna_subway_image.png