Support

Support Options

  • Knowledge Base

    Find information on common questions and issues.

  • Support Messages

    Check on the status of your correspondences with members of the QUBES team.

Contact Us

About you
About the problem

Neochromosomes: Creating transcriptional units with yeast Golden Gate assembly

By Eric Cooper

Hartwick College

This resource is a series of protocols using the yeast Golden Gate (yGG) method as developed by Neta Agmon and Leslie Mitchell in Jef Boeke’s lab to assemble a functional yeast gene with protein-coding and regulatory sequences.

Listed in Teaching Materials | resource by group Build A Genome Network

Download Bundle

We're sorry! Resource content is currently unavailable.

Version 1.0 - published on 28 Oct 2019 doi:10.25334/E2QM-VZ22 - cite this Last public release: 2.0

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

pic.jpg

Description

This resource is a series of protocols using the yeast Golden Gate (yGG) method as developed by Neta Agmon and Leslie Mitchell in Jef Boeke’s lab to assemble a functional yeast gene with protein-coding and regulatory sequences.  First, there are protocols to assemble a single gene whose activity is easy to measure (e.g. an auxotrophic marker, antibiotic resistance gene, LacZ, etc.) just to get the system up and running in your lab.  Once you do, you can apply the methodology to any gene(s) of interest (i.e. it does not have to be a yeast gene).  You can also use a variation of this method to string together multiple genes (via VEGAS assembly) into a single plasmid to study metabolic pathways.

Contents

Cite this work

Researchers should cite this work as follows:

Tags

Build A Genome Network

Build A Genome Network group image

When watching a resource, you will be notified when a new version is released.