Resources
Protein Analysis
Author(s): John Jungck1, Annelise Myers1, Srebrenka Robic2, Gerry Shaw3
1. Beloit College 2. Agnes Scott College 3. University of Florida - Gainesville
113 total view(s), 63 download(s)
Protein_Analysis_4.xls(XLS | 1 MB)- License terms
Overview
This workbook allows the analysis of sample or imported protein sequences. The model can analyze protein sequences up to 500 amino acids long. The program analyzes five aspects of the protein sequence: the highest potential charge along the protein sequence, the amino acid composition of the protein sequence, the isoelectric point of the protein sequence at varying pHs, the hydrophobicity to predict surface and membrane spanning regions of the protein sequence and the protein structure using the Chau and Fassman algorithm.
Popular Text Citations
"Protein Hydrophobicity Plots." (1998) Colorado State University. (Snapshot: 2006-06-17)
King, M.W., 2005. IU School of Medicine. (Snapshot: 2007-12-06)
White, S. (2002) "Experimentally determined hydrophobicity scales." From The Stephen White Laboratory at UC Irvine. Accessed 1 July 2005.
Research Articles
Hopp, T.P.; Woods, K.R. (1981) Prediction of protein antigenic determinants from amino acid sequences. Immunology, 78:3824-3828.
Jungck, J.R. (1978) The Genetic Code as a Periodic Table. Journal of Molecular Evolution, 11: 211-224.
Education Research & Pedagogical Materials
Shaw, G., (1998). 'Spreadsheets in Molecular Biology'. In Spreadsheets in Science and Engineering. Springer-Verlag, Berlin, Pages 203-228.
Shaw, G., (1995). Protein sequence interpretation using a spreadsheet program. BioTechniques, Volume 19, Pages 978-983.
Citation
Researchers should cite this work as follows:
- Jungck, J., Myers, A., Robic, S., Shaw, G. (2024). Protein Analysis. ESTEEM, QUBES Educational Resources. doi:10.25334/TZ7S-1778
Fundamental Mathematical Concepts
Developed By
Primary Reference
Kyte J and Doolittle RF: A simple method for displaying the hydropathic character of a protien. J Mol Biol 157:105, 1982.