| Bio-Rad's Out of the Blue Kits |
"Apply CRISPR-Cas9 technology to disrupt the lacZ gene in E. coli and produce a phenotype that can be readily visualized through blue-white screening." "Confirm the edit at the molecular level using PCR and agarose gel electrophoresis." |
| miniPCR bio's Chopped! Using CRISPR/Cas9 to cut DNA |
"They will use two different guide RNAs to direct Cas9 nuclease to cut a DNA sample at two different locations and then analyze their resulting DNA fragments using gel electrophoresis." |
| miniPCR bio's Knockout! A CRISPR/Cas Gene Targeting Lab |
"In this lab, E. coli cells which express the lacZ gene from a plasmid will be transformed with another plasmid containing the cas9 gene and a custom guide RNA. This guide RNA contains a sequence complementary to the lacZ gene so, in transformed cells, the guide RNA will target Cas9 to cut the lacZ gene, disabling it. Students can easily identify their CRISPR/Cas knockouts using the color of the bacterial colonies." |
| Lab-Aids, Inc.'s The Power of CRISPR |
"Students learn how CRISPR works and how it could be used to treat sickle cell disease, a genetic blood disorder. Students conduct an investigation using CRISPR to edit bacterial genes to study the relationship between genes, proteins, and traits, and also explore supporting information they find in program videos and articles. Students later discuss the ethical aspects of gene editing along with the risks and benefits of using CRISPR for different applications." |
