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    Using Synthetic Biology and pClone Red for Authentic Research on Promoter Function: Introductory Biology (identifying new promoters)
    Students often memorize the definition of a transcriptional promoter but fail to fully understand the critical role promoters play in gene expression. This laboratory lesson allows students to conduct original research by identifying and...

    Courses: GeneticsGenetics Introductory BiologyIntroductory Biology Science Process SkillsScience Process Skills

    bacteriasynthetic biologygene regulationtranscriptionstudent researchcentral dogmacloningpromoterreporter gene/protein
    Lesson
    3089

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    738

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    Using Synthetic Biology and pClone Red for Authentic Research on Promoter Function: Genetics (analyzing mutant promoters)
    Students often memorize the definition of a transcriptional promoter but fail to fully understand the critical role promoters play in gene expression.  This laboratory lesson allows students to conduct original research by characterizing...

    Courses: GeneticsGenetics Science Process SkillsScience Process Skills

    bacteriasynthetic biologygene regulationtranscriptionstudent researchcentral dogmacloningpromoterreporter gene/protein
    Lesson
    1678

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    184

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    Nanoparticles and Shrimp: An Interdisciplinary Lab Series in Chemistry and Biology for Undergraduate Engineering Students
    The parallels between engineering and science are vast, as both disciplines apply theory and inquiry to solve complex problems. In order to prepare students for interdisciplinary careers in STEM, we have developed a lab project in which...

    Courses: Cell BiologyCell Biology EcologyEcology Science Process SkillsScience Process Skills

    microscopychemistry applications in biologytoxicity
    Lesson
    2561

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    641

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    Follow the Sulfur: Using Yeast Mutants to Study a Metabolic Pathway
    Students are frequently overwhelmed by the complexity of metabolic pathways and they think they have "learned" the pathway when they have memorized the individual reactions.  This laboratory lesson helps students to understand the...

    Courses: Biochemistry and Molecular BiologyBiochemistry and Molecular Biology GeneticsGenetics Introductory BiologyIntroductory Biology MicrobiologyMicrobiology Science Process SkillsScience Process Skills

    SelectionyeastMetabolismcysteinemediamethioninemutantPathwaysulfur
    Lesson
    3357

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    1180

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    CRISPR/Cas9 in yeast: a multi-week laboratory exercise for undergraduate students
    Providing undergraduate life-science students with a course-based research experience that utilizes cutting-edge technology, is tractable for students, and is manageable as an instructor is a challenge. Here, I describe a multi-week lesson plan...

    Courses: Biochemistry and Molecular BiologyBiochemistry and Molecular Biology BioinformaticsBioinformatics GeneticsGenetics MicrobiologyMicrobiology Science Process SkillsScience Process Skills

    bioinformaticsPCRphenotypingCRISPR/Cas9gene editingcloningGenotypingRestriction enzyme
    Lesson
    3882

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    209

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    Isolation and Functional Analysis of a Pancreatic Enzyme in an Introductory Student Lab
    Structure and function are correlated at all levels of biology. This topic is typically addressed early in an undergraduate class in general or molecular biology before students have gained much skill or knowledge in molecular biology. However, an...

    Courses: Biochemistry and Molecular BiologyBiochemistry and Molecular Biology Introductory BiologyIntroductory Biology

    Protein StructureproteaseEnzymechemical bondscatalysissolubility
    Lesson
    3653

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    3319

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    Using CRISPR-Cas9 to teach the fundamentals of molecular biology and experimental design
    One of the challenges of inquiry-based research experiences is balancing the instruction on skills and techniques with data generation and analysis. This is especially true in technique heavy disciplines such as molecular biology, where emphasis...

    Courses: Biochemistry and Molecular BiologyBiochemistry and Molecular Biology

    experimental designcrisprmolecular technique
    Lesson
    375

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    60

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    0

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    Environmental Injustice: When the Grass is Greener on the Other Side
    Environmental pollution is a global threat that is especially prevalent in heavily industrialized and urbanized areas. Pollution can be found in many forms, such as natural and synthetic pollutants from natural and anthropogenic processes. These...

    Courses: EcologyEcology Introductory BiologyIntroductory Biology Science Process SkillsScience Process Skills ToxicologyToxicology

    human healthenvironmental injusticeEnvironmental PollutionSystemic BiasesIdeological AwarenessUrban Ecology and Development
    Lesson
    3550

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    263

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    SEAS CURE: Exploring Coral Biology Across Scales
    Complex biological concepts (like symbiosis and coral biology) that span multiple scales and cross disciplinary boundaries are often difficult for students to understand. This complexity is compounded by the challenges inherent to teaching and...

    Courses: Anatomy-PhysiologyAnatomy-Physiology Cell BiologyCell Biology EcologyEcology Introductory BiologyIntroductory Biology Science Process SkillsScience Process Skills

    microscopyAnatomySymbiosisMetabolismbleachinginvertebratedinoflagellate
    Lesson
    3786

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    1247

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    My Dog IS My Homework: Exploring Canine Genetics to Understand Genotype-Phenotype Relationships
    To facilitate understanding of the fundamental genetic concept of the genotype-phenotype relationship in our introductory biology students, we designed an engaging multi-week series of related lessons about canine genetics in which students...

    Courses: BioinformaticsBioinformatics GeneticsGenetics Introductory BiologyIntroductory Biology Science Process SkillsScience Process Skills

    gel electrophoresissequence analysiscanine geneticsdeoxyribonucleic acid (DNA)dog coat morphologygenotype and phenotypemutationsPolymerase chain reaction (PCR)single nucleotide polymorphism (SNP)
    Lesson
    3356

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    270

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    Pesticides in My Smoothie Bowl?
    Teaching resources, especially active learning pedagogy, are scarce for toxicology compared to what is available for other disciplines. Ecological and human health risk assessment are important aspects of toxicology and are routinely used by...

    Courses: ToxicologyToxicology

    data analysistoxicologychemical toxicityecological risk assessmenthuman risk assessmentpesticide
    Lesson
    2091

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    288

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    Assessing in vivo Antimicrobial Activity Through the Analysis of Galleria mellonella Kaplan-Meier Plots
    Antimicrobials and their use in the treatment of bacterial infections are a fundamental lesson in all microbiology courses. Typically, cultivation-based methods are used to teach students about antimicrobial testing. A complementary approach...

    Courses: MicrobiologyMicrobiology

    Antimicrobial TestingAntimicrobialsInvertebrate animal models
    Lesson
    4974

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    460

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    The Avocado Lab: An Inquiry-Driven Exploration of an Enzymatic Browning Reaction
    Typical biochemistry labs exploring basic enzyme activity rely on costly, time-consuming protein purification and rarely explore enzyme function in situ. Further, complex purification procedures leave little room for novelty in experimental...

    Courses: Biochemistry and Molecular BiologyBiochemistry and Molecular Biology Introductory BiologyIntroductory Biology

    enzymescontrolsenzyme inhibition
    Lesson
    1613

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    445

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    Using Structured Decision Making to Explore Complex Environmental Issues
    Environmental issues are inherently complex, often requiring multiple interested parties to come together before agreeing on a solution. Structured decision making is a tool used by federal and state management agencies to find solutions that...

    Courses: EcologyEcology

    interdisciplinarysocio-ecological systemsscience policystakeholderstructured decision making
    Lesson
    1369

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    124

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    Glycolysis Can Be Fun: Rediscovering Glycolysis as a Problem-Solving Introduction to Metabolism
    A thorough understanding of glycolysis forms a foundation for students to analyze subsequent topics in metabolism, a core competency recognized by multiple national societies for biology and biochemistry. However, when confronted with the names of...

    Courses: Biochemistry and Molecular BiologyBiochemistry and Molecular Biology Cell BiologyCell Biology

    Problem based learningguided inquiryenzymesGlycolysisMetabolismFermentationchemical reactionsCard sorting activityCofactors
    Lesson
    1452

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    155

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    The Developmental Origins of Mythology: A Pandemic-Inspired Innovative Developmental Biology Laboratory Exercise
    From an experiential, hands-on perspective, the Developmental Biology Laboratory is easily amenable to a wide range of undergraduate-friendly experiments. Thus, pivoting to a virtual laboratory during the COVID-19 pandemic required significant...

    Courses: Developmental BiologyDevelopmental Biology

    gastrulationaxis formationlimb developmentchimeramidline
    Lesson
    722

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    141

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    Evaluating Representation in Science Through a Peer-Reviewed Research Study
    The demographic representation of scientists featured in biology curricular materials do not match that of the undergraduate biology student population or of the U.S. population. In this lesson, we promote awareness of inequity in science through...

    Courses: Introductory BiologyIntroductory Biology

    societyRepresentationSTEM equity
    Lesson
    4807

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    770

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    Teaching Cancer Biology Through a Lens of Social Justice
    The biology classroom is not separate from the greater context of society; social issues can and should be presented in connection with the content. Here we present an example of antiracist teaching using the molecular/cellular biology of cancer...

    Courses: Cell BiologyCell Biology

    assessmentcancersocial justicePOGILAlignmentPublic ExamPublic
    Lesson
    987

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    182

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    1

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    From Cre/LoxP to Fate Maps: Inclusive and Equitable Approaches for Engaging Developmental Biology Students in Experimental Design
    Engaging first generation underrepresented minority students in the process of inquiry in developmental biology is important to increase diversity in future graduate STEM education. One important challenge is how we design curricula to foster...

    Courses: Developmental BiologyDevelopmental Biology

    experimental designC. elegansCre/LoxPFate mapheart developmentheart regenerationmousereporter constructzebrafish
    Lesson
    3686

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    200

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    Three Research-Based Quantitative Reasoning Modules for Introductory Organismal Biology Laboratories
    We have designed three laboratory modules for an introductory organismal biology course with an emphasis on quantitative reasoning and data analysis skills. Module 1 tests for dimorphism in crayfish chelae using a paired statistical design. Module...

    Courses: Introductory BiologyIntroductory Biology Science Process SkillsScience Process Skills

    allometryplantsAnatomytranspirationdimorphisminvertebrate biologyarthropodsplatyhelminths