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    Using Synthetic Biology and pClone Red for Authentic Research on Promoter Function: Genetics (analyzing mutant promoters)
    Students often memorize the definition of a transcriptional promoter but fail to fully understand the critical role promoters play in gene expression.  This laboratory lesson allows students to conduct original research by characterizing...

    Courses: GeneticsGenetics Science Process SkillsScience Process Skills

    bacteriasynthetic biologygene regulationtranscriptionstudent researchcentral dogmacloningpromoterreporter gene/protein
    Lesson
    3358

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    1180

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    CRISPR/Cas9 in yeast: a multi-week laboratory exercise for undergraduate students
    Providing undergraduate life-science students with a course-based research experience that utilizes cutting-edge technology, is tractable for students, and is manageable as an instructor is a challenge. Here, I describe a multi-week lesson plan...

    Courses: Biochemistry and Molecular BiologyBiochemistry and Molecular Biology BioinformaticsBioinformatics GeneticsGenetics MicrobiologyMicrobiology Science Process SkillsScience Process Skills

    bioinformaticsPCRphenotypingCRISPR/Cas9gene editingcloningGenotypingRestriction enzyme
    Lesson
    2973

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    612

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    Using Synthetic Biology and pClone Red for Authentic Research on Promoter Function: Introductory Biology (identifying new promoters)
    Students often memorize the definition of a transcriptional promoter but fail to fully understand the critical role promoters play in gene expression. This laboratory lesson allows students to conduct original research by identifying and...

    Courses: GeneticsGenetics Introductory BiologyIntroductory Biology Science Process SkillsScience Process Skills

    bacteriasynthetic biologygene regulationtranscriptionstudent researchcentral dogmacloningpromoterreporter gene/protein
    Lesson
    3657

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    3320

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    Using CRISPR-Cas9 to teach the fundamentals of molecular biology and experimental design
    One of the challenges of inquiry-based research experiences is balancing the instruction on skills and techniques with data generation and analysis. This is especially true in technique heavy disciplines such as molecular biology, where emphasis...

    Courses: Biochemistry and Molecular BiologyBiochemistry and Molecular Biology

    experimental designcrisprmolecular technique
    Teaching Tools and Strategies
    1737

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    326

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    SCIENCE/Fiction: Modular Template for Class Design
    We present a modular scaffold for designing an introductory seminar using speculative fiction and science papers to welcome university undergraduates to the biology major. A list of twelve possible topics is included, such as Memory, Parasites,...

    Courses: Anatomy-PhysiologyAnatomy-Physiology Biochemistry and Molecular BiologyBiochemistry and Molecular Biology Cell BiologyCell Biology EvolutionEvolution GeneticsGenetics Introductory BiologyIntroductory Biology NeurobiologyNeurobiology

    geneticscell biologyneurosciencesystems biologyScience Fiction
    Lesson
    3291

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    548

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    A Student-Led Hearing on the Use of HeLa Cells in Research
    In this role-playing case study, students will learn about the HeLa cell line and its origins while examining multiple perspectives surrounding the cell line and its uses. The goal of this case study is for students to use guided questions to...

    Courses: Cell BiologyCell Biology Science Process SkillsScience Process Skills

    bioethicsscience policycell culturedebateHeLa cellsinformed consentrole playsocial justice issues
    Lesson
    1598

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    344

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    Investigating Gene Expression and Cell Specialization in Axolotl Embryos
    The process of cell specialization is critical to the formation and function of tissues in animals and plants. Although gene expression, including the regulation of transcription, is taught in most introductory cell biology courses, the...

    Courses: Biochemistry and Molecular BiologyBiochemistry and Molecular Biology Cell BiologyCell Biology Developmental BiologyDevelopmental Biology Science Process SkillsScience Process Skills

    developmenttranscriptiongene expressionmodel organismembryo
    Lesson
    3506

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    1299

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    A CRISPR/Cas Guide RNA Design In Silico Activity
    CRISPR biotechnologies inspired by the Clustered Regularly Interspaced Short Palindromic Repeat RNA-guided nuclease adaptive bacterial immune system have revolutionized biology research and become ubiquitous tools for hypothesis and...

    Courses: Biochemistry and Molecular BiologyBiochemistry and Molecular Biology BioinformaticsBioinformatics Cell BiologyCell Biology GeneticsGenetics

    bioinformaticsgene expressiongenetic basis of diseaseCRISPR/Cas9genetic engineeringin silico
    Lesson
    2196

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    459

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    Antibiotic Resistance Genes Detection in Environmental Samples
    Our aim is to provide an authentic research experience for undergraduate students in a variety of biology courses through collaboration between a four-year university and a community college. This lab series teaches transferable and universal...

    Courses: Introductory BiologyIntroductory Biology MicrobiologyMicrobiology Science Process SkillsScience Process Skills

    geneticsenvironmentmicrobiologyUndergraduateantibiotic-resistancePCRgel electrophoresisauthentic inquiry