Wilson Leung created this post
GFP Tagging Module
This module was developed by Douglas Chalker, Washington University, MO. Students engineer genetic constructs for the tagging and expression of a Tetrahymena protein of interest. Fluorescence microscopy is used to determine localization of the tagged protein. (Estimated time: 9 x 4 hour laboratory periods.)
The Ciliate Genome Consortium enables student participation in the functional annotation of genes from Tetrahymena thermophila. Students determine the functions of Tetrahymena genes through localization, expression, and gene knockout or over-expression studies. These research activities are packaged into modules to facilitate their integration into the undergraduate biology curriculum.