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Bioinformatics Module I
This lab is the beginning of a 12-week project during which students will clone a gene from the ciliate Tetrahymena thermophila, fuse it to the gene encoding GFP, put the engineered gene back into Tetrahymena, and induce its expression. The gene will be cloned by PCR, so the first step is to design primers to allow the amplification of the desired gene. This lab will take students through the steps to do that, and will also demonstrate the use of other bioinformatics tools.
The Ciliate Genome Consortium enables student participation in the functional annotation of genes from Tetrahymena thermophila. Students determine the functions of Tetrahymena genes through localization, expression, and gene knockout or over-expression studies. These research activities are packaged into modules to facilitate their integration into the undergraduate biology curriculum.