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    Cell Culture Best Practices: Getting Started
    These video resources show how to get started working in the lab.

    Keywords: beginnerchemical hygiene and lab safety

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    Growing Lung Carcinoma and Lung Fibroblast Cell Lines
    Basic information on how to grow Lung Carcinoma and Lung Fibroblast Cell Lines.

    Keywords: tipsCell Growthcell cultureA-549HFL-1

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    Easy Hypoxia Treatments for Cell Culture a CBEC Cell Block
    A combination of ideas we have found from various sources. These can be used in an undergraduate lab or Cell CURE.

    Keywords: hypoxia

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    Scratch Assay (Cell Migration) Cell Block
    A video tutorial and pedagogy notes describing how to measure cell migration using a wound/scratch assay. This is an inexpensive and relatively easy experiment. A micropublication that used this...

    Keywords: wound healingcourse-based research experiences (CUREs)Cell culture-based researchScratch AssayCell migrationCancer cell biology

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    Getting Started: Drug Treatment Calculations
    This assignment will help teach students...

    Keywords: experimentMicropipettingcalculationsdrug calculation

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    Developing a semester long cancer cell culture CURE
    A weekly outline for developing a semester-long cell culture CURE. Includes examples of student class presentations with annotated notes.

    Keywords: cell biologyPresentationcourse-based research experiences (CUREs)Cell culture-based researchCBECSemester outline

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    How to Grow Specific Cell Lines: Liver Cancer Cell Lines (SNU-423 and Hep-G2)
    General information for growing cell lines. One is a high-grade (fast) cancer the other is a low-grade. Makes for great comparisons within a class setting.

    Keywords: tipsCell GrowthHepG2SNU-423 ATCC

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    Setting up PC12 Differentiation Assay Cell Block
    How to conduct a differentiation assay using PC12 cells. Complete video protocol.

    Keywords: neurobiologycourse-based research experiences (CUREs)neuorscienceCell culture-based researchPC12

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    Hemocytometer and Counting Cells Cell Block
    This resource describes how to count cells using a hemocytometer.

    Keywords: lab protocolcounting cells

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    Migration Assay Protocol
    Migration is a key property of live cells and critical for normal development, immune response, and disease processes such as cancer metastasis and inflammation.

    Keywords: migration assay

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    MTT Assay Video Protocol
    This assay works great. However, the cells are killed during the course of the assay. If you want cells to remain alive use the Alamar Blue assay.

    Keywords: MTT Assay

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    Journal Reading: Day 1 in the Lab
    Done on the first day of lab, choose a research article or a review article related to the theme of the class.

    Keywords: Labclass projectsPC12

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    Fatty Acid Induction of Lipid Droplets in Cancer Cells
    There is a growing need for the development and communication of cell culture-based laboratory activities specifically designed for undergraduate students. This multi-week laboratory activity...

    Keywords: Course based undergraduate researchCell ImagingLive Cell Staining

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    Using a Primary Cell Culture Model to Study the Neural Extracellular Matrix
    The extracellular matrix (ECM) provides structural support to cells, but also has key roles in mediating cell adhesion, cell signaling, and differentiation. While this topic is discussed in the...

    Keywords: Neural extracellular matrixPerineuronal netPrimary neuronal culture

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    A virtual laboratory on cell division using a publicly-available image database
    Cell division is a key concept in cell biology. While there are many popular activities to teach students the stages of mitosis, most make use of simple schematics, cartoons, or textbook diagrams....

    Keywords: image analysismicroscopymitosisCellular Processes

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    A Structured Inquiry-Based Module for the Undergraduate Cell Biology Laboratory That Teaches Fundamental Concepts of Cell Differentiation
    Pedagogical research in science education has shown that students effectively learn science by doing science. As a result, there is increased interest in bringing research-like experiences into the...

    Keywords: fluorescence microscopygene expressionpolymerase chain reactionCell Structurecell culturewestern blottingdifferentiationreverse transcription

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    CBEC - Cell Biology Education Consortium (RCN-UBE Introduction)
    The CBEC helps faculty at PUIs move cancer cell culture research into the classroom. The mechanism that provides faculty with the resources they need to do this are modular protocols called Cell...

    Keywords: RCN-UBE Introduction

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    iEMBER - Novel Networking Fostering Interdisciplinary Communities and New Research Collaborations (RCN-UBE Introduction)
    The vision of the inclusive Environments and Metrics in Biology Education and Research (iEMBER) Network is to improve biology education for learners that are often traditionally excluded from the...

    Keywords: RCN-UBE Introduction

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    Microbiomes forAll: The Research Experiences in Microbiomes Network
    Poster on REMNet presented at the 2020 BIOME Institute: Cultivating Scientific Curiosity

    Keywords: undergraduate researchcourse-based undergraduate research experiencemicrobiomesmicrobiomeCUREREMNet

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    Using Yeast to Make Scientists: A Six-Week Student-Driven Research Project for the Cell Biology Laboratory
    Traditionally-trained undergraduate students often lack an understanding of science as an active process that yields the information presented in their textbooks. One result has been a call for...

    Keywords: yeastgrowth curvecell proliferationviability

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