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    Setting up PC12 Differentiation Assay Cell Block
    How to conduct a differentiation assay using PC12 cells. Complete video protocol.

    Keywords: PC12

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    Scratch Assay Cell Block
    A video and tutorial describing how to compete a scratch assay.

    Keywords: Scratch Assay

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    Migration Assay Protocol
    Migration is a key property of live cells and critical for normal development, immune response, and disease processes such as cancer metastasis and inflammation.

    Keywords: migration assay

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    Protein Import Into the ER: Understanding Experimental Methods for Old and New Cellular Discoveries
    Process Oriented Guided Inquiry Learning (POGIL) is an effective approach for group work in STEM courses. POGIL-style lessons foster active learning because they require students to communicate and...

    Keywords: Necessary and sufficientsignal sequenceProtein SynthesistrafficER importTranslocation

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    MTT Assay Video Protocol
    This assay works great. However, the cells are killed during the course of the assay. If you want cells to remain alive use the Alamar Blue assay.

    Keywords: MTT Assay

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    Osmosis Through the Lens of Sexual Health
    Osmosis contributes to a range of biological phenomena, from functioning of nephrons in animals to stomatal opening and closing in plants; yet, scientifically simple ideas about osmosis are common....

    Keywords: OsmosisDiffusionsexual healthsocial and cultural embeddedness of scienceinclusive dialogue

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    A Journal Club Project to Promote Interpreting and Presenting Information From the Primary Scientific Literature
    Many undergraduate students receive little guidance on how to critically read, interpret data within, or present information from the primary scientific literature before they begin senior...

    Keywords: primary literaturejournal clubScience CommunicationfeedbackPeer reviewgroup workOral presentationProcess skillsPeer evaluationDevelopmental neurobiology

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    A Structured Inquiry-Based Module for the Undergraduate Cell Biology Laboratory That Teaches Fundamental Concepts of Cell Differentiation
    Pedagogical research in science education has shown that students effectively learn science by doing science. As a result, there is increased interest in bringing research-like experiences into the...

    Keywords: fluorescence microscopygene expressionpolymerase chain reactionCell Structurecell culturewestern blottingdifferentiationreverse transcription

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    Breaking It Down: What Factors Control Microbial Decomposition Rates?
    Demonstrating and modeling changes in ecosystem processes in the laboratory classroom can be logistically difficult and expensive. This complexity often leaves little time for students to generate...

    Keywords: data analysishypothesis testingdata visualizationdata managementMicrobial RespirationDecompostionAbiotic ConditionsResults Communication

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    Understanding the Importance of Microtubules in Normal Cell Division: Chemotherapy Connection
    In introductory courses, students learn about microtubules as important structures but may not engage in a hands-on experience to localize microtubules themselves or to learn about their connection...

    Keywords: cancerCell DivisionimmunofluorescenceMicrotubulesAntibodies

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    The Power of Place: A Course-Based Undergraduate Research Experience Studying Urban Ecology, Local Apple Trees and Disease Susceptibility
    Course-based Undergraduate Research Experiences (CUREs) offered early in an undergraduate student’s career have the potential to include a greater number and diversity of students than...

    Keywords: Disease ecologyurban ecologyUrban food forestsUrban heat islandsGenetic resistance

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    An Interactive Protocol for In-Classroom DNA Extraction
    Using commonly available materials, this tool allows students to extract DNA, exploring DNA chemistry and the principles of experimental design and execution. We take a “Choose Your Own...

    Keywords: geneticsDNAbiochemical structure and function

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    BSL-4: Authorized Personnel Only
    National Center for Case Study Teaching in Science resource on the 2014 Ebola epidemic. This resource is also included in the NIBLSE Learning Resource Collection

    Keywords: infectious diseaseexperimental designbioinformaticsEbolaNIBLSECase It!PCRBLASTmultiple sequence alignmentPublished in National Center for Case Study Teaching in Sciencepolymerase chain reactionfebrile illnessvirusELISAenzyme linked immunosorbent assayfilovirusAfrica

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    Garden Variety Mutations: Using Primary Data to Understand the Central Dogma in Large-Lecture Introductory Biology
    The ability to interpret and create an argument from data is a crucial skill for budding scientists, yet one that is seldom practiced in introductory courses. During this argumentation module,...

    Keywords: mutationtranscriptiongene expressioncentral dogmatranslationgel electrophoresisGenotype/Phenotype

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    Isolation and Functional Analysis of a Pancreatic Enzyme in an Introductory Student Lab
    Structure and function are correlated at all levels of biology. This topic is typically addressed early in an undergraduate class in general or molecular biology before students have gained much...

    Keywords: Protein StructureproteaseEnzymechemical bondscatalysissolubility

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    Application of a Bacterial Experimental Evolution System to Visualize and Teach Evolution in Action: A Course-Based Undergraduate Research Experience
    Concepts of evolution are typically taught through examples of extremely long timescales, which do not always resonate broadly. Here, we describe a course-based undergraduate research experience...

    Keywords: EvolutionmutationcompetitionNatural Selectionregulationstructure and functionreproductive fitnessgenotype to phenotypehomologs

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    "I Really Enjoy These Annotations:" Examining Primary Biological Literature Using Collaborative Annotation
    Critically reading and evaluating claims made in the primary literature are vital skills for the future professional and personal lives of undergraduate students. However, the formal presentation...

    Keywords: Stem cellsAdult neurogenesisCRISPR gene editing

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    Does Organelle Shape Matter?: Exploring Patterns in Cell Shape and Structure with High-Throughput (HT) Imaging
    Organelle structure has been studied and visualized for decades; however, publicly available databases that use improved high-throughput microscopy of gene-edited cell lines have recently...

    Keywords: modelingcase studymicroscopystructureOrganellesHigh-throughput (HT)Endoplasmic Reticulum (ER)Fluorescent microscopyHuman Cell Biology

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    A 360˚ View of COVID-19
    In March 2020, institutions underwent a massive transition to distance learning as a result of the COVID-19 pandemic. With so little time to devise new materials to maximize learning in the new...

    Keywords: geneticsImmunologyBiochemistrybioinformaticsmolecular evolutionpublic healthvirusCOVID19

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    Learning About Protein Localization: A Lesson for Analyzing Figures in a Scientific Publication
    In order to function correctly, proteins must be localized to a specific subcellular location. We have designed this lesson to use data from the primary literature to teach students about the...

    Keywords: colocalizationimmunofluorescenceProtein localizationsignal sequencessubcellular fractionationwestern blotting

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