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    Growing Lung Carcinoma and Lung Fibroblast Cell Lines
    Basic information on how to grow Lung Carcinoma and Lung Fibroblast Cell Lines.

    Keywords: tipsCell Growthcell cultureA-549HFL-1

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    Easy Hypoxia Treatments for Cell Culture a CBEC Cell Block
    A combination of ideas we have found from various sources. These can be used in an undergraduate lab or Cell CURE.

    Keywords: hypoxia

    110

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    How to Grow Specific Cell Lines: Liver Cancer Cell Lines (SNU-423 and Hep-G2)
    General information for growing cell lines. One is a high-grade (fast) cancer the other is a low-grade. Makes for great comparisons within a class setting.

    Keywords: tipsCell GrowthHepG2SNU-423 ATCC

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    Scratch Assay Cell Block
    A video and tutorial describing how to compete a scratch assay.

    Keywords: Scratch Assay

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    Hemocytometer and Counting Cells Cell Block
    This resource describes how to count cells using a hemocytometer.

    Keywords: lab protocolcounting cells

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    Setting up PC12 Differentiation Assay Cell Block
    How to conduct a differentiation assay using PC12 cells. Complete video protocol.

    Keywords: PC12

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    Migration Assay Protocol
    Migration is a key property of live cells and critical for normal development, immune response, and disease processes such as cancer metastasis and inflammation.

    Keywords: migration assay

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    MTT Assay Video Protocol
    This assay works great. However, the cells are killed during the course of the assay. If you want cells to remain alive use the Alamar Blue assay.

    Keywords: MTT Assay

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    Journal Reading: Day 1 in the Lab
    Done on the first day of lab, choose a research article or a review article related to the theme of the class.

    Keywords: Labclass projectsPC12

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    CBEC - Cell Biology Education Consortium (RCN-UBE Introduction)
    The CBEC helps faculty at PUIs move cancer cell culture research into the classroom. The mechanism that provides faculty with the resources they need to do this are modular protocols called Cell...

    Keywords: RCN-UBE Introduction

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    Using a Primary Cell Culture Model to Study the Neural Extracellular Matrix
    The extracellular matrix (ECM) provides structural support to cells, but also has key roles in mediating cell adhesion, cell signaling, and differentiation. While this topic is discussed in the...

    Keywords: Neural extracellular matrixPerineuronal netPrimary neuronal culture

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    Fatty Acid Induction of Lipid Droplets in Cancer Cells
    There is a growing need for the development and communication of cell culture-based laboratory activities specifically designed for undergraduate students. This multi-week laboratory activity...

    Keywords: Course based undergraduate researchCell ImagingLive Cell Staining

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    A virtual laboratory on cell division using a publicly-available image database
    Cell division is a key concept in cell biology. While there are many popular activities to teach students the stages of mitosis, most make use of simple schematics, cartoons, or textbook diagrams....

    Keywords: image analysismicroscopymitosisCellular Processes

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    A Structured Inquiry-Based Module for the Undergraduate Cell Biology Laboratory That Teaches Fundamental Concepts of Cell Differentiation
    Pedagogical research in science education has shown that students effectively learn science by doing science. As a result, there is increased interest in bringing research-like experiences into the...

    Keywords: fluorescence microscopygene expressionpolymerase chain reactionCell Structurecell culturewestern blottingdifferentiationreverse transcription

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    Using Immunocytochemistry and Fluorescence Microscopy Imaging to Explore the Mechanism of Action of Anti-Cancer Drugs on the Cell Cycle
    Understanding and visualizing the cell cycle and mitosis is an essential concept of cell biology. This multi-week lab module has students investigate the mechanism of action of anti-cancer drugs...

    Keywords: fluorescence microscopymitosisCell StructureCell ImagingImmunocytochemistryQuantitative microscopySTEM skills

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    Using Yeast to Make Scientists: A Six-Week Student-Driven Research Project for the Cell Biology Laboratory
    Traditionally-trained undergraduate students often lack an understanding of science as an active process that yields the information presented in their textbooks. One result has been a call for...

    Keywords: yeastgrowth curvecell proliferationviability

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    From Dirt to Streptomyces DNA
    The purpose of this semester-long Lesson is to give students an authentic, course-based undergraduate research experience during which they learn basic and advanced microbiological and molecular...

    Keywords: MetabolismAntibiotic productionrRNA genesSecondary metabolitesStreptomyces

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    E. coli Citrate Use
    EvoEd module on citrate use in E.coli

    Keywords: modulephylogeneticsecologycell biologymolecular genetics

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    Re-Envisioning Culture Network: A Syllabus Guide
    The Re-Envisioning Culture Network Syllabus aims to honor Black identity and culture while acknowledging the barriers that Black undergraduate students face through definitions, art, storytelling,...

    Keywords: stemraceAddressing anti-blacknessBlack in STEM

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    Going Remote: An Online Adaptation to Using a Primary Cell Culture Model to Study the Neural Extracellular Matrix
    In light of the COVID-19 pandemic, instructors have modified materials to transition to a remote learning platform. The challenge for science instructors has been to design lab exercises that...

    Keywords: remote learningExtracellular matrixNeural extracellular matrixPerineuronal net

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