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#1904, v1.0 Published:
#1918, v1.0 Published:

Title

Old VersionNew Version
1Understanding COVID-19 Biology to Design a Vaccine 1COVID-19: On the way to a vaccine

Authors

Old VersionNew Version
1Keith Johnson () 1Nik Tsotakos ()
2Didem Vardar-Ulu (Boston University) 2Nik Tsotakos ()
3Shuchismita Dutta ()   
4Keith Johnson ()   

Description

Old VersionNew Version
1<p>The case presented here is written during the COVID-19 pandemic (Spring 2020) and uses this backdrop to introduce students to the initial stages of developing a vaccine against the SARS-CoV-2 virus, targeting its spike protein.&nbsp; The viral protein binds to a specific human protein on human epithelial cells and this initiates the infection. The structure of the viral spike and human receptor protein interaction was&nbsp; determined using cryo-electron microscopy by Yan <em>et al</em> (2020). Using the freely available molecular structural data and publicly available visualization software and bioinformatics tools and resources, the case leads students to examine the interaction of the viral spike (S) protein with the human epithelial cell ACE-2 protein, the site of viral attachment. The vaccine development discussed in this case is focused on blocking this interaction to stop infection.</p>  1<p>The case presented here is written during the COVID-19 pandemic (Spring 2020) and uses this backdrop to introduce students to a complete set of studies in virology. The students will understand how the virus evolved, and what its relationship is to SARS and MERS, two other coronaviruses responsible for past epidemics. They will understand the molecular basis of diagnosis, and design their own diagnostic kit, as well as understand the infection process, and explore the genetics of the human receptors for SARS-CoV-2. The structure of the viral spike and human receptor protein interaction was&nbsp; determined using cryo-electron microscopy by Yan <em>et al</em> (2020). Using the freely available molecular structural data and publicly available visualization software and bioinformatics tools and resources, the case leads students to examine the interaction of the viral spike (S) protein with the human epithelial cell ACE-2 protein, the site of viral attachment. The vaccine development discussed in this case is focused on blocking this interaction to stop infection.</p>
2    
3<p>&nbsp;</p>    
4    
5<p>Painting depicting coronavirus. (Acknowledgement: Illustration by David S. Goodsell, RCSB Protein Data Bank; doi: 10.2210/rcsb_pdb/goodsell-gallery-019.)</p>   

Attachments

1 file — KeithJohnson/Johnson et al COVID-19 answer key-6180.docx 1 file — COVID-19_Resources-20200613.docx
2 file — KeithJohnson/Johnson et al COVID-19 resources.docx 2 file — NikTsotakos/Part I - Alignment.docx
3 file — KeithJohnson/Johnson et al COVID-19 teaching notes.docx 3 file — NikTsotakos/Part II - Diagnostic kit.docx
4 file — KeithJohnson/Johnson et al COVID-19 worksheet 1.docx 4 file — NikTsotakos/Parts III-IV - Genetics and physiology.docx
5 file — KeithJohnson/Johnson et al COVID-19 worksheet 2.docx 5 file — NikTsotakos/TeachingNotes.docx
6 file — KeithJohnson/Johnson et al COVID-19 worksheet 3.docx 6 file — Part V - Molecular visualization.docx
7 file — KeithJohnson/Johnson et al COVID-19 worksheet 4.docx 7 file — Part VI - Comparison of S proteins.docx
8 file — KeithJohnson/Johnson et al COVID-19 worksheet 5 assessment.docx 8 link — Clustal Omega < EMBL-EBI
9 file — KeithJohnson/Johnson et al Worksheet 2 ethics supplement.docx 9 link — RCSB PDB: Homepage
10 file — KeithJohnson/coronavirus.png 10 link — GTEx Portal
11 link — OMIM - Online Mendelian Inheritance in Man
12 link — Human Cell Atlas
13 link — UCSC Genome Browser Home
14 link — Virus Pathogen Database
15 link — MEGA software homepage
16 file — publication_2060_2074/KeithJohnson/coronavirus.png
17 link — iCn3D: Web-based 3D Structure Viewer
18 link — Gene - NCBI
19 file — NikTsotakos/2019-nCoV qPCR.pdf
20 file — NikTsotakos/rt-pcr-panel-primer-probes.pdf