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#1907, v1.0 Published:
#1925, v1.0 Published:

Title

Old VersionNew Version
1Nicholas' Story 1Molecular Basis of Sickle Cell Disease

Authors

Old VersionNew Version
1Kimberly Linenberger Cortes (Kennesaw State University) 1Didem Vardar-Ulu ()
2Shuchismita Dutta () 2Didem Vardar-Ulu ()
3Henry Vincent Jakubowski (College of Saint Benedict/Saint John's University)   
4Melanie Lenahan (Raritan Valley Community College)   
5David Marcey (California Lutherian University)   
6Pat Marsteller (Emory University)   
7Cassidy Terrell (University of Minnesota, Rocester)   
8Shuchismita Dutta ()   

Description

Old VersionNew Version
1<p><a href="https://molecular-casenet.rcsb.org/node/12">Nicholas&#39; Story</a></p>  1<p><a href="https://molecular-casenet.rcsb.org/node/12">Molecular Basis of Sickle Cell Disease</a></p>
2  2  
3<p><em>Overview</em>: This case discusses Nicholas&#39; experiences with sickle cell disease. The case begins with watching a video where Nicholas and his mother, Bridget, talk about living with sickle cell disease. The case is organized into three sections that explores the molecular basis of Nicholas&#39; pain crises, and mechanisms of action and implications of his current treatment with a small molecule drug, called hydroxyurea. It also discusses novel approaches being used and developed to treat sickle cell disease.</p>  3<p><em>Overview</em>: This case is an adaptation of the Nicholas&#39;s story that discusses Nicholas&#39; experiences with sickle cell disease.&nbsp; Background materials about the original case are included in the pre-class worksheet along with additional information and exercises.&nbsp;The in-class section is divided into three parts that contain&nbsp;the core of the molecular visualization exercises.&nbsp; The first part focuses on understanding oxyen binding through using the RCSB Protein Data Bank resources as well as the&nbsp;<i>Online Macromolecular Museum </i>exhibit.&nbsp; Second part focuses on developing a molecular understanding of sickle cell disease using the&nbsp;the&nbsp;<i>Online Macromolecular Museum </i>exhibit.&nbsp; The last part &#39;s designed to have students discuss in molecular terms the cause of Nicholas&#39;s pain and propose&nbsp; interventions that would prevent pain crises.&nbsp;&nbsp;</p>
4  4  
5<p><em>Learning Objectives</em>: The case was developed at the interface of Biology and Chemistry to enable introductory biology students explore chemical interactions that stabilize the structure and enable functions of biological molecules; and introductory chemistry students in applying their knowledge of intra- and inter-molecular forces to authentic biological contexts. The case can even be used to teach advanced students concepts in biochemistry, cell and molecular biology, such as oxygen binding, drug design, and gene therapy approaches to treat sickle cell disease. There is flexibility in depth of content coverage and range of additional related topics and molecules that can be included in the discussions. By the end of the case, students should develop some basic understanding of bio-molecular structure-function relationships.</p>  5<p><em>Learning Objectives</em>: This adaptation was developed for an upper level one semester introductory biochemistry course without a laboratory taken primarily by premed students.&nbsp; As a chemistry cause the emphasis is on the molecular details and the students are expected to be familiar with basic protein structure and noncovalent interactions to best benefit from the activity.&nbsp;&nbsp;&nbsp; By the end of the case, students should be able to:</p>
6  6  
7<p><em>Molecules explored</em>: The primary molecule studied in this case is hemoglobin, including structures of native, mutant, and variant proteins; and complexes with drugs and various small molecular ligands.</p>  7<ul>
   8   <li>retrieve protein sequences from public repositories</li>
   9   <li>obtain basic information about the protein sequences using open license software</li>
   10   <li>align and compare protein sequences using open license software</li>
   11   <li>predict secondary structure of proteins based on sequence using open license software</li>
   12   <li>use RCSB Protein Data Bank to retrieve structural information about proteins whose 3D structure is deposited in the databank</li>
   13   <li>explore molecular interactions by visualizing available 3D protein structures with freely available online tools</li>
   14   <li>explain the molecular basis of oxygen binding to hemoglobin and the structural changes associated with oxygen binding</li>
   15   <li>explain the molecular basis of sickle cell disease and the structural changes associated with the single point mutation underlying the sickle cell disease</li>
   16</ul>
8  17  
9<p><em>Implementation</em>: The case can be implemented using either a flipped approach and/or in-class discussions.</p> 18<p><em>Molecules explored</em>: The primary molecule studied in this case is hemoglobin, including structures of native, mutant, and variant proteins; and complexes with drugs and various small molecular ligands.&nbsp; Students are also asked to look into myoglobin during their pre-class work as a preparation for the case.</p>
   19
   20<p><em>Implementation</em>: The case is designed to be delivered in&nbsp;an hybrid approach with pre- and post- sections&nbsp;implemented using a flipped approach in-class section carried out in person.&nbsp; However, each section can be delivered with the other modality as well.</p>

Attachments

1 link — Nicholas' Story 1 file — DidemVardar-Ulu/Grading rubric for In-class work.pdf
2 file — NicholasStory.png 2 file — DidemVardar-Ulu/In-class test_Gradescope format.pdf
3 file — DidemVardar-Ulu/In_class work_KEY.pdf
4 file — DidemVardar-Ulu/In_class work_Student Version.pdf
5 file — DidemVardar-Ulu/Post-class work Version B_KEY and grading rubric.pdf
6 file — DidemVardar-Ulu/Post-class work_Version A KEY and grading rubric.pdf
7 file — DidemVardar-Ulu/Post-class work_Version A.pdf
8 file — DidemVardar-Ulu/Post-class work_Version B.pdf
9 file — DidemVardar-Ulu/Pre_class work_KEY.pdf
10 file — DidemVardar-Ulu/Pre_class work_Student version.pdf
11 file — DidemVardar-Ulu/TeachingNotes-Spr2020_DVU.pdf
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