| 1 | <p><a href="https://molecular-casenet.rcsb.org/node/19">Piwi Matters</a></p>
| 1 | <p><span calibri="" light="">In this adaptation of the case study, <em>Piwi Matters</em>, mid- to upper-level molecular biology students learn how bacteria use CRISPR/Cas9 as an immune system. In addition, students learn how scientists have adapted CRISPR/Cas9 to be used to make mutations in model organisms. By using published protocols, students design gRNA target sequences and a single-strand donor template to create the mutation that they designed in <em>Piwi Matters</em>. Alternatively instructors can have students design mutations of other genes. Finally, students present their intended mutation, gRNA target, and donor template in 2-4 minute oral presentations. Ultimately, the class uses these presentations to vote which mutation should be made. </span></p> |
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| 3 | <p><em>Overview</em>: Stem cells have the ability to self renew and differentiate. This case discusses how these properties of germline stem cells are regulated by a protein called Piwi, that is highly conserved in many species. Presence and function of this protein is required for producing large numbers of eggs in fruit flies. The RNA binding ability of this protein is critical for its function. The case begins with watching a video about a researcher in this field describing how the role of Piwi was identified and is currently studied to understand its structure-function relationships.</p>
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| 5 | <p><em>Learning Objectives</em>: The case was developed for introductory genetics courses where students are introduced to the molecular structural basis for <span>ovary development. Students learn to identify and access data from various biological databases and integrate information to understand the structure and functions of this protein. </span>The explorations may be expanded to a laboratory exercise where students can develop hypothesis about Piwi's structure-function relationships and design experiments to test them.</p>
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| 7 | <p><em>Molecules explored</em>: Molecular structures of Piwi complexes are explored to understand structure function relationships.</p>
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| 9 | <p><em>Implementation</em>: The case can be implemented using either a flipped approach and/or in-class discussions. Detailed teaching notes, mini lessons, and discussion prompts for open ended discussions are available for download to guide the in-class activity.</p> | | |
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