Resources: Compare

#165, v2.0 Published:
#599, v1.0.0 Published:

Title

Old VersionNew Version
1Using DNA Subway to Analyze Sequence Relationships 1Intro to DNA Subway Blue Line

Authors

Old VersionNew Version
1Elizabeth F Ryder (Worcester Polytechnic Institute) 1Oliver Hyman (The Department of Biology, James Madison University; The Center for Genome & Metagenome Studies, James Madison University )
   2Andrea Pesce (James Madison University)
   3Emily Lescak ()
   4William Tapprich (University of Nebraska-Omaha)
2Jason Williams (DNA Learning Center) 5Jason Williams (DNA Learning Center) 
3William Tapprich (University of Nebraska-Omaha) 3Elizabeth Ryder (Worcester Polytechnic Institute)
4Emily Lescak (University of Alaska) 4Ray A Enke (The Department of Biology, James Madison University; The Center for Genome & Metagenome Studies, James Madison University )
5Oliver Hyman (The Department of Biology, James Madison University; The Center for Genome & Metagenome Studies, James Madison University )   
6Ray A. Enke (The Department of Biology, James Madison University; The Center for Genome & Metagenome Studies, James Madison University )   
7Sam S Donovan (University of Pittsburgh) 8Sam S Donovan (University of Pittsburgh) 
8Hayley Orndorf (University of Pittsburgh) 9Hayley Orndorf (University of Pittsburgh) 
  10Hayley Orndorf () 
  11Elizabeth Doyle (James Madison University) 

Description

Old VersionNew Version
1<p>These are instructions for a 120-minute bioinformatics lab in which students will learn how to use the DNA Subway (<a href="http://www.dnasubway.org">http://www.dnasubway.org</a>) Blue Line. DNA Subway is an online educational bioinformatics platform. It bundles research-grade bioinformatics tools, high-performance computing, and databases into workflows with an easy-to-use interface. The Blue Line is a workflow for analyzing DNA bar-coding data to determine the taxonomic identity of an organism and examine inferred phylogenetic relationships among species. The data analyzed in this exercise is a collection of mosquito DNA sequences generated by students at James Madison University. Identification of a mosquito&rsquo;s genus is important because different mosquitoes are capable of carrying different pathogens causing deadly human diseases.</p>  1<p>Materials include:</p>
2  2  
3<p>Note: A natural way to divide this material may be to cover everything up to section IV (Phylogenetic Trees) in one class, leaving additional time to address trees in as much depth as desired.</p> 3<ol>
   4   <li>A lab activity (with answers included) introducing DNA Barcoding, DNA Subway Blue Line and a short Blue Line activity (HYMAN et al. 2016 DNA Subway Blue Line Lab KEY.docx)</li>
   5   <li>A electronic lab notebook to help students record the data they generate in Blue Line (HYMAN et al. DNA subway lab notebook entry.docx)</li>
   6   <li>Two slide decks that help guide students through DNA Subway Blue Line. The first slide deck is a self-guided tour for students to use on their own (HYMAN et al. DNA Subway_Independent Blue Line Practice.pptx). The second slide deck is designed for an instructor led tour in class (HYMAN et al. DNA Subway_Instructor led Blue Line Practice.pptx).&nbsp;</li>
   7   <li>A slide deck designed to help instructors deal with common issues that can arise during blue line analyses (HYMAN et al. worked example slides for instructors.pptx).</li>
   8   <li>Two &quot;unknown&quot; Sanger sequencing reads (.ab1 files) for the Blue Line activity.</li>
   9</ol>

Attachments

1 file — ./Jason Williams - barcoding_intro.pptx 1 file — ./Hyman Adaptation/1-plant-pos-fall15F_PREMIX_J43474_3.ab1
2 file — ./Using-DNA-Subway-to-Analyze-Sequence-Barcodes-05182018-Instructor.docx 2 file — ./Hyman Adaptation/1-plant-pos-fall15R_PREMIX_J43475_4.ab1
3 file — ./Using-DNA-Subway-to-Analyze-Sequence-Barcodes-05182018-Student.docx 3 file — ./Hyman Adaptation/HYMAN et al. 2016 DNA Subway Blue Line Lab KEY.docx
4 file — ./dna_subway_image.png 4 file — ./Hyman Adaptation/HYMAN et al. DNA subway lab notebook entry.docx
5 file — ./Hyman Adaptation/HYMAN et al. DNA Subway_ Independent Blue Line Practice.pptx
6 file — ./Hyman Adaptation/HYMAN et al. DNA Subway_ Instructor led Blue Line Practice.pptx
7 file — ./Hyman Adaptation/HYMAN et al. worked example slides for instructors.pptx
8 file — publication_647_677/dna_subway_image.png