This authentic research experience lesson teaches the core concept of systems and the competencies of quantitative reasoning, communication, and the ability to apply science. The research is student driven, the results are unknown, and the students engage in an iterative process to gather data, collaborating with classmates.  It is designed for first-year biology majors, in a class size of 15-30 students who can work in groups of three.  Students will learn to properly design an experiment, work as teams, analyze data, evaluate conclusions, and communicate findings to others. Additionally, this lesson also incorporates self-reflection and peer assessment when students produce a poster as a summative assessment. Over a five–week period, students will explore how an abiotic factor affects growth, reproduction, and survival of Daphnia.  Students are asked to compare their results to published literature. By the end, students should have a better understanding of science as an ongoing process where results are being updated and furthering the state of knowledge.

Beginning undergraduate students in biology need basic laboratory, data analysis, and science process skills to pursue more complex questions in course-based undergraduate research experiences (CUREs). To this end, we designed an introductory lesson that helps students learn to use common laboratory equipment such as analytical balances and micropipettes, analyze and present data in Google and Microsoft spreadsheet software, and perform simple descriptive and inferential statistics for hypothesis testing. In this lesson, students first learn to use micropipettes by pipetting specific volumes of water correctly and incorrectly. After determining the masses of the water samples pipetted, students enter the data into a shared Google spreadsheet and then use statistics to test a null hypothesis; ultimately, they determine if there is a statistically significant difference between the mass of water pipetted correctly versus incorrectly. Together, these activities introduce students to important data analysis and science process skills while also orienting them to basic laboratory equipment.

A challenge in introductory biology laboratory courses is to provide students with authentic, engaging research opportunities that allow them to take ownership of their experiments. We present a nine-week introductory biology module that allows students to engage with the process of science, gain experience with various laboratory techniques, and communicate their results to a peer audience. These modules use the inexpensive and accessible invertebrate model of the brine shrimp Artemia, which has many applications from aquaculture to ecology to behavior. Students explore known taxis behaviors in the larval (or “naupliar/nauplii”) stages of these brine shrimp before designing their own experiments, collecting and analyzing data, presenting their results orally, and redesigning their experiments based on peer and instructor feedback. This LessonPlus article highlights the exploration of known taxis behaviors and the scaffolding for having students design their own experiments. We originally designed this module to be highly flexible and used it to teach students both remotely and in-person during the early years of the pandemic. We have since found it to be easily adaptable in terms of timing, materials used, and learning modality. Most importantly, we have observed a number of positive outcomes related to student engagement and proficiency, including increased quality of summative assessments.

Primary Image: Artemia nauplius. A scientific illustration of the nauplius stage of Artemia sp. used in these experiments to study taxis behaviors. ©2024 Liesl V. McCormick.