"Genomics/Evolution" 3 posts Sort by created date Sort by defined ordering View as a grid View as a list

A Fun Introductory Command Line Lesson: Next Generation Sequencing Quality Analysis with Emoji!

Radical innovations in DNA sequencing technology over the past decade have created an increased need for computational bioinformatics analyses in the 21st century STEM workforce. Recent evidence however demonstrates that there are significant barriers to teaching these skills at the undergraduate level including lack of faculty training, lack of student interest in bioinformatics, lack of vetted teaching materials, and overly full curricula. To this end, the James Madison University, Center for Genome & Metagenome Studies (JMU CGEMS) and other PUI collaborators are devoted to developing and disseminating engaging bioinformatics teaching materials specifically designed for streamlined integration into general undergraduate biology curriculum. Here, we have developed and integrated a fun introductory level lesson to command line next generation sequencing (NGS) analysis into a large enrollment core biology course. This one-off activity takes a crucial but mundane aspect of NGS quality control (QC) analysis and incorporates the use of Emoji data outputs using the software FASTQE to pique student interest. This amusing command line analysis is subsequently paired with a more rigorous research-grade software package called FASTP in which students complete sequence QC and filtering using a few simple commands. Collectively, this short lesson provides novice-level faculty and students an engaging entry point to learning basic genomics command line programming skills as a gateway to more complex and elaborated applications of computational bioinformatics analyses.

Primary image: Undergraduate students learn the basics of command line NGS quality analysis using the FASTQE and FASTP programs.

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Bárbara Bitarello onto Genomics/Evolution

Introduction to nucleotide sequence analysis and protein modeling in MEGA and PyMol using coronavirus SARS-CoV-2

Introduction into computational approaches in phylogeny and protein modeling based on coronavirus SARS-CoV-2 (caused COVID-19 pandemic). Two self-guided tutorials for standard lab classes of 2.5 hours. Level: undergraduate students majoring in biology.

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Bárbara Bitarello onto Genomics/Evolution

Studying phylogeny by producing phylogenetic trees of primates using morphological and molecular characteristics.

An assignment constructing phylogenetic trees using shared, derived morphological traits and molecular differences among 18 primate species and two outlier species has been developed. Skull and body images and a table of morphological characteristics were used to fill in a pre-fabricated tree. Molecular differences of the cytochrome b gene obtained from the NCBI website was used to generate a tree using the Phylogeny.fr website for the same 20 species. Several followup questions were provided upon completion of the assignment.

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Bárbara Bitarello onto Genomics/Evolution