In order to introduce students to the concept of molecular diversity, we developed a short, engaging online lesson using basic bioinformatics techniques. Students were introduced to basic bioinformatics while learning about local on-campus species diversity by 1) identifying species based on a given sequence (performing Basic Local Alignment Search Tool [BLAST] analysis) and 2) researching and documenting the natural history of each species identified in a concise write-up. To assess the student’s perception of this lesson, we surveyed students using a Likert scale and asking them to elaborate in written reflection on this activity. When combined, student responses indicated that 94% of students agreed this lesson helped them understand DNA barcoding and how it is used to identify species. The majority of students, 89.5%, reported they enjoyed the lesson and mainly provided positive feedback, including “It really opened my eyes to different species on campus by looking at DNA sequences”, “I loved searching information and discovering all this new information from a DNA sequence”, and finally, “the database was fun to navigate and identifying species felt like a cool puzzle.” Our results indicate this lesson both engaged and informed students on the use of DNA barcoding as a tool to identify local species biodiversity.

Primary Image: DNA Barcoded Specimens. Crane fly, dragonfly, ant, and spider identified using DNA barcoding.

Speciation provides a framework for classifying biodiversity on Earth and is a central concept in evolutionary biology. To help undergraduate students learn about speciation, we designed a student-centered lesson that uses active-learning techniques (e.g., clicker questions, small group work, and whole class discussion) and compares multiple species concepts (morphological, biological, and phylogenetic) using giraffes as an example. Giraffes were chosen as the focus of this lesson because they are familiar and have broad appeal to students; are in danger of becoming extinct; and have ecological, economic, and cultural importance. Students also learn about contemporary giraffe conservation issues and the current debate in the literature regarding the total number of giraffe species. Students then apply their knowledge by working in small groups on speciation scenarios that highlight organisms across the tree of life. Student understanding is assessed using multiple-choice pre/post-test questions, in-class clicker questions with peer discussion, and exam questions. Here we provide details about the lesson and report that student learning is improved.