This module teaches about the three key steps that are involved in converting the pre-mRNA into a mature mRNA: 1) The addition of a 5’ cap, 2) The addition of a 3’ poly(A) tail, 3) The removal of introns through splicing.
This module demonstrates how the transcript generated by RNA polymerase II (the pre-mRNA) is processed to become mature mRNA using the sequence signals identified in Module 2. After completing this module, students will be able to use the genome browser to explain the relationships among pre-mRNA, 5' capping, 3' polyadenylation, splicing, and mRNA. The specific nucleotide sequences that guide the maturation process are highlighted. Students will observe that introns are removed during this process and adjacent exons are brought together in the mRNA message.
- Module_3_Cover_Page.docx(DOCX | 32 KB)
- Module_3_Cover_Page.pdf(PDF | 72 KB)
- Module_3_Transcription_Part_II.docx(DOCX | 2 MB)
- Module_3_Transcription_Part_II.pdf(PDF | 1 MB)
- Module_3_Transcription_Part_II_Answer_Sheet.docx(DOCX | 42 KB)
- Module_3_Transcription_Part_II_Answer_Sheet.pdf(PDF | 28 KB)
- Module 3: Transcription, Part II: What Happens to the Initial Transcript Made by RNA pol II? (Read The Docs Version)
- License terms
Cite this work
Researchers should cite this work as follows:
- Key, C. S., Small, C., Reed, L. (2020). Module 3: Transcription, Part II: What Happens to the Initial Transcript Made by RNA pol II?. Genomics Education Alliance, QUBES Educational Resources. doi:10.25334/PYZN-JQ45