Support

Support Options

  • Knowledge Base

    Find information on common questions and issues.

  • Support Messages

    Check on the status of your correspondences with members of the QUBES team.

Contact Us

About you
About the problem

Module 4: Removal of Introns from pre-mRNA by Splicing

By Meg Laakso1, Anne Rosenwald2

1. Eastern University 2. Georgetown University

In this module, students will learn to identify splice donor and acceptor sites that are best supported by RNA-Seq data, and use the canonical splice donor and splice acceptor sequences to identify intron-exon boundaries.

Listed in Teaching Materials | resource by group Genomics Education Alliance

Version 1.0 - published on 25 Jul 2020 doi:10.25334/030X-Q219 - cite this

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

Figure01.png Figure02.png Figure04.png Figure05.png Figure07.png

Description

All RNAs in the cell are collectively known as the "transcriptome" as almost all RNA is produced by transcription from a DNA template. (In some cases, RNA is made from an RNA template.) The transcriptome includes messenger RNAs, ribosomal RNAs, transfer RNAs, and other RNAs that have specialized functions in the cell. Here, we will investigate how mRNA specifically is modified. Students will learn to identify splice donor and acceptor sites that are best supported by RNA-Seq and TopHat splice junction predictions and use the canonical splice donor and splice acceptor sequences to identify intron-exon boundaries.

Contents

Cite this work

Researchers should cite this work as follows:

Tags

Genomics Education Alliance

Genomics Education Alliance group image

When watching a resource, you will be notified when a new version is released.