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Functional and structural analysis of Amylase activity

By Ishrat Rahman1, Aubrey Allen Smith1, Evdokia Kastanos2

1. Montgomery College 2. Montgomery College/Rockville

This exercise was developed to allow students to apply the scientific method and use their knowledge of enzymes to investigate the relationship between enzyme activity and structure. Students design experiments to study the effect of...

Listed in Teaching Materials

Version 1.0 - published on 23 Nov 2020 doi:10.25334/VKGJ-VT46 - cite this

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

Description

Upon completion of this exercise, the student should be able to:

  1. design experiment(s) with appropriate controls to test their hypothesis.
  2. make a standard curve for estimation of maltose concentration.
  3. set up enzyme-catalyzed (amylase) hydrolysis of starch into maltose.
  4. explain the relationship between amount of maltose produced and the activity of amylase.
  5. obtain amino acid sequences of amylase from the NCBI online database.
  6. use bioinformatics tools to analyze primary structures, compare amino acid sequences and generate a 3D structure of each enzyme.

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