Understanding Restriction Enzyme Digests
Author(s): Swati Agrawal1, Sara Anderson2, Dustin Edwards3, Bryan Gibb4, Matthew David Mastropaolo5
1. University of Mary Washington 2. Minnesota State University Moorhead 3. Tarleton State University 4. New York Institute of Technology 5. Neumann University
1099 total view(s), 1222 download(s)
Summary:
Prior to submitting genomic DNA for sequencing, SEA students perform a restriction endonuclease digest with several enzymes, which provides a fast and cost-effective way of quickly screening isolated phages. The pattern of bands on the gel should be…
Contents:
- 0 - Facilitator Guide Restriction Enzyme Digest 1.pdf(PDF | 76 KB)
- 1A - Primary Literature Review.pdf(PDF | 33 KB)
- 1B - Naming of Restriction Enzymes.pdf(PDF | 27 KB)
- 1KEY - Primary Literature Review.pdf(PDF | 53 KB)
- 2 - Paper Restriction Enzyme Activity.pdf(PDF | 262 KB)
- 2KEY - Paper Restriction Enzyme Activity.pdf(PDF | 273 KB)
- 3 - Digital Lambda-HindIII Digest.pdf(PDF | 219 KB)
- 3KEY - Digital Lambda-HindIII Digest.pdf(PDF | 96 KB)
- 4 - Virtual Digest of Phage Genomes.pdf(PDF | 2 MB)
- 4KEY - Virtual Digest of Phage Genomes.pdf(PDF | 41 KB)
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