Yeast Scramble Lab Manual v2
Author(s): Mark Jones, John Mirarchi1, Lisa Scheifele1
Loyola University Maryland
182 total view(s), 390 download(s)
Summary:
This laboratory manual includes protocols for SCRaMbling of yeast cells as well as phenotypic characterization. Appropriate student exercises are also included. This resource was developed as part of NSF Award #1827204, RCN-UBE: The Build-a-Genome…
Contents:
- Yeast Scramble Lab Manual v2.docx(DOCX | 2 MB)
- Yeast Scramble Lab Manual v2.pdf(PDF | 818 KB)
- License terms
Description
The Synthetic Yeast Project Sc2.0 developed synthetic yeast chromosomes with LoxPsym sites scattered throughout the chromosomes. By inducing the expression of Cre recombinase, new chromosome configurations can be generated in yeast cells. This population can then be screened for enhanced or altered phenotypes.
Notes
This second version includes more details in the protocols to clarify pre-lab preparation, supply lists and media recipes, and protocols for analysis of next-generation (Illumina) sequencing data.
Cite this work
Researchers should cite this work as follows:
- Jones, M., Mirarchi, J., Scheifele, L. (2022). Yeast Scramble Lab Manual v2. Build A Genome Network, QUBES Educational Resources. doi:10.25334/11X6-QD82