Evaluating the potential for immune escape: how likely is an antibody to protect against a specific SARS-CoV-2 variant?
Author(s): Allyson Sterling1, Erica Lannan2, Sandra Porter3
1. Regis University 2. Prairie State College 3. Digital World Biology
170 total view(s), 234 download(s)
- Instructions.docx(DOCX | 4 MB)
- Worksheet ANSWER KEY.docx(DOCX | 2 MB)
- https://NextStrain.org
- National Center for Biotechnology Information
- SARS CoV 2 variants vs Antibodies - YouTube
- iCn3D: Web-based 3D Structure Viewer
- SAbDab: The Structural Antibody Database
- SARS-CoV-2 Abs vs Variants Feb2022.pptx(PPTX)
- License terms
Description
This resource updates the 2022 version with new workflows from iCn3D and contains the following items:
1. An Instructor resource describing some tips for using this resource with students, including changing the pathogen of interest.
2. A Student guide describing different parts of the activity, including a worksheet and answer key.
There are five parts to the activity. The first three are optional and last two parts are the main research project. The optional parts (1, 2, and 3) describe how students can compare conformations of the spike protein receptor binding domain (part 1), find aligned models from different structures (part 2), and identify a protein binding site (part 3).
The research component consists of finding a protein sequence from a variant (part 4) and comparing that sequence to an older version of the spike protein to evaluate how mutations may impact antibody binding (part 5).
Each part is accompanied by a worksheet for recording experimental data. The data sheets also make it easier for an instructor to assess the quality of the work because students have some guidance concerning the type of information that needs to recorded.
Lastly, a PowerPoint presentation is provided that goes through the research project and a video that illustrates the technical components.
This work was funded by the National Science Foundation under Grant No. (DUE 2055036). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.
Cite this work
Researchers should cite this work as follows:
- Sterling, A., Lannan, E., Porter, S. (2024). Evaluating the potential for immune escape: how likely is an antibody to protect against a specific SARS-CoV-2 variant?. Antibody Engineering, (Version 2.0). QUBES Educational Resources. doi:10.25334/Q7KG-5V20