This interactive assignment engages students in a hands-on exploration of microbial diversity and the importance of microbes in ecosystems. Designed for General Biology II and Introduction to Microbiology courses, the activity accommodates class sizes of 10-30 students. Within a 55-minute class period, students work in groups to research, analyze, and present various microorganisms. This guide includes detailed steps for instructors, a student worksheet, a PowerPoint presentation with microbial images, and quizzes to assess student learning.