Intro to DNA Subway Blue Line
Author(s): Oliver Hyman1, Andrea Pesce2, Elizabeth Doyle2, Ray A Enke1
1. The Department of Biology, James Madison University; The Center for Genome & Metagenome Studies, James Madison University 2. James Madison University
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Summary:
This is a classroom tested introductory lesson & activity to introduce DNA Barcoding and DNA Subway Blue Line. It is for novices and will fit into a 1 hr 50 min lecture or lab class period.
Contents:
Bundle(ZIP | 31 MB)- License terms
Description
Materials include:
- A lab activity (with answers included) introducing DNA Barcoding, DNA Subway Blue Line and a short Blue Line activity (HYMAN et al. 2016 DNA Subway Blue Line Lab KEY.docx)
- A electronic lab notebook to help students record the data they generate in Blue Line (HYMAN et al. DNA subway lab notebook entry.docx)
- Two slide decks that help guide students through DNA Subway Blue Line. The first slide deck is a self-guided tour for students to use on their own (HYMAN et al. DNA Subway_Independent Blue Line Practice.pptx). The second slide deck is designed for an instructor led tour in class (HYMAN et al. DNA Subway_Instructor led Blue Line Practice.pptx).
- A slide deck designed to help instructors deal with common issues that can arise during blue line analyses (HYMAN et al. worked example slides for instructors.pptx).
- Two "unknown" Sanger sequencing reads (.ab1 files) for the Blue Line activity.
Cite this work
Researchers should cite this work as follows:
- Hyman, O., Pesce, A., Doyle, E., Enke, R. A. (2018). Intro to DNA Subway Blue Line. QUBES Educational Resources. doi:10.25334/Q48H7N