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Build-a-Phage Lab Manual

Author(s): Lisa Scheifele1, Eric Cooper2, Robert Newman3

1. Loyola University Maryland 2. Hartwick College 3. North Carolina A&T University

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Summary:
This is a lab manual for phage genome synthesis that includes both Building Block synthesis and Semi-synthetic assembly.

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

Version 1.0 - published on 25 Jul 2019 doi:10.25334/8VBB-4A95 - cite this

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Description

This resource is a lab protocols manual for the "Build-a-Phage" workflow. This is one of the three workflows for Build-a-Genome, a course-based undergraduate research experience (CURE) in which students edit and manipulate genomes to learn about genome structure and function.  This course, which was designed to align with many of the goals of Vision and Change, allows students to conduct authentic, interdisciplinary research in the cutting edge field of synthetic biology by designing, building, and testing the function of a selected genome.  The lab manual includes protocols for an initial boot-camp phase (“Building Block assembly”) in which students learn the techniques by assembling a model gene such as green or red fluorescent protein. Students then proceed to use these same techniques for the assembly of semi-synthetic phage genomes. Student assignments and a materials supply list are also included.

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