Picking a Workflow

Build-a-Genome supports 3+ workflows. For each, we have lab procedures and protocols that are ready to go as well as longer-term research projects which we are happy to have you join!



Ready-to-go workflow 

Ongoing research project

(be sure to be logged in to QUBEShub to view!)

Synthesis of Red Fluorescent Protein

Build-a-Genome Lab Manual for RFP Synthesis

Build-a-Genome Lab Manual for RFP Synthesis

Lisa Scheifele

Version: 1.0

This lab manual describes a multi-week lab project for the design, assembly from oliognucleotides, cloning, and sequencing of the RFP gene. This resource was developed as part of NSF Award #1827204, RCN-UBE: The Build-a-Genome Network.
introductory, Lab, Teaching material, Undergraduate, Advanced, Majors, Extended Project

Build-a-Phage Lab Manual

Build-a-Phage Lab Manual

Lisa Scheifele, Eric Cooper, Robert Newman

Version: 1.0

This is a lab manual for phage genome synthesis that includes both Building Block synthesis and Semi-synthetic assembly.
Lab, Teaching material, Reference material, Undergraduate, Extended Project, Workshop material

Join us on the Mycobacteriophage Giles engineering project to make a fully synthetic and modular phage genome that can be used for subsequent modification and hypothesis testing. Contact is Lisa Scheifele


Neochromosomes: Creating transcriptional units with yeast Golden Gate assembly

This resource is a series of protocols using the yeast Golden Gate (yGG) method as developed by Neta Agmon and Leslie Mitchell in Jef Boeke’s lab to assemble a functional yeast gene with protein-coding and regulatory sequences.
Lab, Teaching material, Undergraduate, Advanced, Extended Project

Join us on the promoter bashing project to use yeast golden gate assembly to probe the structure and function of yeast promoters. Contact is Eric Cooper

Scramble (Yeast Genome Rearrangement)

Yeast Scramble Lab Manual

Yeast Scramble Lab Manual

Mark Jones, Lisa Scheifele

Version: 1.0

This laboratory manual includes protocols for SCRaMbling of yeast cells as well as phenotypic characterization. Appropriate student exercises are also included. This resource was developed as part of NSF Award #1827204, RCN-UBE: The Build-a-Genome Network
introductory, Lab, Teaching material, Undergraduate, Advanced, Majors, Extended Project

Join us on the BUGSS Yeast Scramble project to combine the beauty of yeast art and Scramble-help us to make new colors in yeast and expand our color palette. Contact is Lisa Scheifele

Teaching ethics in synthetic biology

Ethics in Synthetic Biology teaching modules

Ethics in Synthetic Biology

Lisa Scheifele, Fran Sandmeier

Version: 3.0

This collection of five activities provide students with an introduction to thinking about ethical concerns in human genome synthesis, gene editing, metabolic engineering, and synthetic genomes.
biostatistics, statistical analysis, avian malaria, Summer Workshop 2018, Poster, Reference material, Abstract, Workshop material

Help us to write, implement, and assess teaching modules on the ethics of synthetic biology! Contact is Fran Sandmeier


Learning Objectives and Assessment

Following our Second Annual workshop in 2019, the network developed the following common learning objectives. Suggested methods for assessing each of the learning objectives are indicated. 

Learning Objective     Assessment Method

    1. Students can explain how engineering principles distinguish synthetic biology from other biological disciplines


    2. Students gain confidence in their experimental skills and better understand the process of conducting research

CURE Survey and/or Build-a-Genome Student Survey

    3. Students can explain the steps of PCR and gene assembly and the role that temperature plays at each of the steps

Lab Assignments

    4. Students can troubleshoot experiments by making rational changes to experimental parameters

PCR Troubleshooting Worksheet

    5. Students can effectively present experimental results in oral (or written) form

Lab Meetings

    6. Students can explain the relationship between genes and genomes, name some non-gene components of a genome, and describe how biological systems or genomes can be manipulated to address practical applications

Essay or Final Report