Picking a Workflow
Build-a-Genome supports 3+ workflows. For each, we have lab procedures and protocols that are ready to go as well as longer-term research projects which we are happy to have you join!
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Ready-to-go workflow |
Ongoing research project (be sure to be logged in to QUBEShub to view!) |
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| Synthesis of Red Fluorescent Protein |
Build-a-Genome Lab Manual for RFP Synthesis Build-a-Genome Lab Manual for RFP SynthesisVersion: 1.0 This lab manual describes a multi-week lab project for the design, assembly from oliognucleotides, cloning, and sequencing of the RFP gene. This resource was developed as part of NSF Award #1827204, RCN-UBE: The Build-a-Genome Network. |
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| Bacteriophages |
Build-a-Phage Lab ManualVersion: 1.0 This is a lab manual for phage genome synthesis that includes both Building Block synthesis and Semi-synthetic assembly. |
Join us on the Mycobacteriophage Giles engineering project to make a fully synthetic and modular phage genome that can be used for subsequent modification and hypothesis testing. Contact is Lisa Scheifele
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| Neochromosomes |
Neochromosomes: Creating transcriptional units with yeast Golden Gate assembly This resource is a series of protocols using the yeast Golden Gate (yGG) method as developed by Neta Agmon and Leslie Mitchell in Jef Boeke’s lab to assemble a functional yeast gene with protein-coding and regulatory sequences. |
Join us on the promoter bashing project to use yeast golden gate assembly to probe the structure and function of yeast promoters. Contact is Eric Cooper.
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| Scramble (Yeast Genome Rearrangement) |
Yeast Scramble Lab ManualVersion: 1.0 This laboratory manual includes protocols for SCRaMbling of yeast cells as well as phenotypic characterization. Appropriate student exercises are also included. This resource was developed as part of NSF Award #1827204, RCN-UBE: The Build-a-Genome Network |
Join us on the BUGSS Yeast Scramble project to combine the beauty of yeast art and Scramble-help us to make new colors in yeast and expand our color palette. Contact is Lisa Scheifele |
| Teaching ethics in synthetic biology |
Ethics in Synthetic Biology teaching modules Ethics in Synthetic BiologyVersion: 3.0 This collection of five activities provide students with an introduction to thinking about ethical concerns in human genome synthesis, gene editing, metabolic engineering, and synthetic genomes. |
Help us to write, implement, and assess teaching modules on the ethics of synthetic biology! Contact is Fran Sandmeier.
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