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Design of gRNA Target and Donor

This adaptation is for an upper level molecular biology laboratory course. In this adaptation, students learn the content required to design a gRNA target sequence and single-strand donor template to make their desired mutation.

Listed in Teaching Materials | resource by group Molecular CaseNet Faculty Mentoring Network

Version 1.0 - published on 16 Jun 2020 doi:10.25334/9XWQ-E437 - cite this

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms


In this adaptation of Piwi Matters, upper level molecular biology laboratory students learn the content to understand how bacteria use CRISPR/Cas9 as an acquired immunity. In addition, students learn how scientists have adapted CRISPR/Cas9 to be used in eukaryotic model organisms. By using published protocols, students design gRNA target sequences and a single-strand donor template to create the mutation that they designed in the Piwi matters case study. Finally, students present the mutation, gRNA target, and donor template in a 2-4 minute oral presentation. Ultimately, the class uses these presentations to vote which mutation should be made.


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