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Summary:
This laboratory manual includes protocols for SCRaMbling of yeast cells as well as phenotypic characterization. Appropriate student exercises are also included. This resource was developed as part of NSF Award #1827204, RCN-UBE: The Build-a-Genome…
Contents:
Yeast Scramble Lab Manual.pdf(PDF | 363 KB)- License terms
Description
The Synthetic Yeast Project Sc2.0 developed synthetic yeast chromosomes with LoxPsym sites scattered throughout the chromosomes. By inducing the expression of Cre recombinase, new chromosome configurations can be generated in yeast cells. This population can then be screened for enhanced or altered phenotypes.
Cite this work
Researchers should cite this work as follows:
- Jones, M., Scheifele, L. (2020). Yeast Scramble Lab Manual. Build A Genome Network, QUBES Educational Resources. doi:10.25334/9N64-QR80