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Yeast Scramble Lab Manual

By Mark Jones, Lisa Scheifele1

Loyola University Maryland

This laboratory manual includes protocols for SCRaMbling of yeast cells as well as phenotypic characterization. Appropriate student exercises are also included.

Listed in Teaching Materials | resource by group Build A Genome Network

Version 1.0 - published on 28 Jul 2020 doi:10.25334/9N64-QR80 - cite this

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

yeast.jpg

Description

The Synthetic Yeast Project Sc2.0 developed synthetic yeast chromosomes with LoxPsym sites scattered throughout the chromosomes. By inducing the expression of Cre recombinase, new chromosome configurations can be generated in yeast cells. This population can then be screened for enhanced or altered phenotypes.

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