This laboratory manual includes protocols for SCRaMbling of yeast cells as well as phenotypic characterization. Appropriate student exercises are also included.
The Synthetic Yeast Project Sc2.0 developed synthetic yeast chromosomes with LoxPsym sites scattered throughout the chromosomes. By inducing the expression of Cre recombinase, new chromosome configurations can be generated in yeast cells. This population can then be screened for enhanced or altered phenotypes.
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