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Understanding Frameshifting

By Rachel Arnold1, Sara Tolsma2, Alyssa Gleichsner3, Margaret Hopson-Fernandes4

1. Northwest Indian College 2. Northwestern College 3. SUNY Plattsburgh 4. Hillsborough Community College

This module contains activities to help students understand the biology of programmed translational frameshifts (PTFs) in phages, how to identify PTFs, and how to annotate them.

Listed in Teaching Materials | resource by group HHMI Science Education Alliance (SEA) Faculty Group

Version 1.0 - published on 25 Sep 2020 doi:10.25334/EFX8-YD76 - cite this

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

Frameshifting Image.png

Description

This module contains activities to help students understand the biology of programmed translational frameshifts (PTFs) in phages, how to identify PTFs, and how to annotate them.

Learning objectives:  

After completing this module, students should be able to:

  • Annotate a programmed translational frameshift
  • Identify the protein products of the FS and the function of the protein products (see “Programmed Translational Frameshifts Powerpoint”)
  • Explain how the ribosome produces protein products from the FS (see “Programmed Translational Frameshifts Powerpoint”)
  • Explain/present the data on PTFS from journal articles in the primary literature (see “Guidelines_for_Journal_Club_Presentation--Students.docx, ”Guidelines_for_Journal_Club_Presentation_WithExample--for_Faculty_with_Assessment.docx" and Frameshift Articles)

How is the module structured to promote student development as a scientist?  Students using activities in this module will use primary literature, find and annotate a PTF, and work with other student scientists.

Intended Teaching Setting

Course level: All levels

Instructional Setting:  In-person or online

Implementation Time Frame:  approximately 3 hours

Acknowledgments:  HHMI, SEA-PHAGES, Hatfull lab at University of Pittsburgh

Project Documents:

Learning activity files:  

Frameshift_identification_activity_Final.docx

Guidelines_for_Journal_Club_Presentation--Students.docx

PECAAN_frameshiftcoding.mp4 (Video File)

Baranov, et al, 2006 paper (DOI:https://doi.org/10.1016/j.tig.2006.01.005)

Caliskan et al., 2015 paper (available free at:  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126180/)

Xu et al, 2004 paper (available free at at:  https://www.cell.com/fulltext/S1097-2765(04)00539-8)

Programmed Translational Frameshift Final Version.ppt  

Assessment document(s): 

Frameshift_identification_Activity_AnswerKey.docx

Guidelines_for_Journal_Club_Presentation_WithExample--for_Faculty_with_Assessment.docx


 

 

 

 

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HHMI Science Education Alliance (SEA) Faculty Group

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