Understanding Frameshifting
Author(s): Rachel Arnold1, Sara Tolsma2, Alyssa Gleichsner3, Margaret Hopson-Fernandes4
1. Northwest Indian College 2. Northwestern College 3. SUNY Plattsburgh 4. Hillsborough Community College
918 total view(s), 801 download(s)
Frameshifting Image.png(PNG | 36 KB)
Guidelines_for_Journal_Club_Presentation--Students.docx(DOCX | 15 KB)- Guidelines_for_Journal_Club_Presentation_WithExample--for_Faculty_with_assessment.docx(DOCX | 17 KB)
- Is There A Frameshift Student Worksheet.docx(DOCX | 688 KB)
- Is There A Frameshift Worsheet Answer Key.docx(DOCX | 40 KB)
PECAAN_frameshiftcoding.mp4(MP4 | 17 MB)
Programmed Translational Frameshiftsfinalversion.pptx(PPTX | 481 KB)- License terms
Description
This module contains activities to help students understand the biology of programmed translational frameshifts (PTFs) in phages, how to identify PTFs, and how to annotate them.
Learning objectives:
After completing this module, students should be able to:
- Annotate a programmed translational frameshift
- SEA-PHAGES Bioinformatics Guide: What is a programmed translational frameshift? (https://seaphagesbioinformatics.helpdocsonline.com/article-6)
- Videos on annotating frameshifts in DNA Master
- Video on annotating a frameshift in PECAAN
- PECAAN_frameshiftcoding.mp4 (Video File included in this set of teaching resources)
- Identify the protein products of the FS and the function of the protein products (see “Programmed Translational Frameshifts Powerpoint”)
- Explain how the ribosome produces protein products from the FS (see “Programmed Translational Frameshifts Powerpoint”)
- Explain/present the data on PTFS from journal articles in the primary literature (see “Guidelines_for_Journal_Club_Presentation--Students.docx, ”Guidelines_for_Journal_Club_Presentation_WithExample--for_Faculty_with_Assessment.docx" and Frameshift Articles)
How is the module structured to promote student development as a scientist? Students using activities in this module will use primary literature, find and annotate a PTF, and work with other student scientists.
Intended Teaching Setting
Course level: All levels
Instructional Setting: In-person or online
Implementation Time Frame: approximately 3 hours
Acknowledgments: HHMI, SEA-PHAGES, Hatfull lab at University of Pittsburgh
Project Documents:
Learning activity files:
Frameshift_identification_activity_Final.docx
Guidelines_for_Journal_Club_Presentation--Students.docx
PECAAN_frameshiftcoding.mp4 (Video File)
Baranov, et al, 2006 paper (DOI:https://doi.org/10.1016/j.tig.2006.01.005)
Caliskan et al., 2015 paper (available free at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126180/)
Xu et al, 2004 paper (available free at at: https://www.cell.com/fulltext/S1097-2765(04)00539-8)
Programmed Translational Frameshift Final Version.ppt
Assessment document(s):
Frameshift_identification_Activity_AnswerKey.docx
Guidelines_for_Journal_Club_Presentation_WithExample--for_Faculty_with_Assessment.docx
Cite this work
Researchers should cite this work as follows:
- Arnold, R., Tolsma, S. S., Gleichsner, A., Hopson-Fernandes, M. (2020). Understanding Frameshifting. HHMI Science Education Alliance (SEA) Faculty Group, QUBES Educational Resources. doi:10.25334/EFX8-YD76