QUBES will have scheduled maintenance work on Tuesday, December 17, 2024, beginning sometime between 5 pm PST and 11:59 pm PST, with downtime lasting approximately 30 minutes. All running tool sessions will expire during the maintenance window. Please plan accordingly and we do apologize for any inconvenience. close

You are here: Home / Resources / Teaching Materials / Analysis of Amylase Activity / Description
Resource Image

Analysis of Amylase Activity

Author(s): Allison Carter Burlyn1, Christina Fieber1, Melanie Lenahan2

1. Horry-Georgetown Technical College 2. Raritan Valley Community College

1479 total view(s), 16703 download(s)

0 comment(s) (Post a comment)

Download

Adapt 0
Brought to you by

Catalytic Graphers

Catalytic Graphers group image

Summary:
This lab is an exploration of enzyme function and the effects of environmental conditions on the activity of the enzyme amylase. The lab utilizes analytical and graphing skills to assess enzyme activity.

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

Version 1.0 - published on 24 Nov 2020 doi:10.25334/CW0W-Y495 - cite this

Keywords

  1. data analysis
  2. introductory biology
  3. concentration
  4. graphing
  5. linear equations
  6. Biochemistry
  7. scientific method
  8. standard curve
  9. enzyme function
  10. Microsoft Excel
  11. Interpreting Graphs
  12. enzymes
  13. qbcc
  14. Create graphs
  15. Interpret graphs
  16. Solving Equations and Inequalities
  17. interpret tables
  18. Amylase
  19. Alpha-amylase
  20. Enzyme activity
  21. Maltose
  22. DNS assay
  23. Absorbance
  24. lab report
  25. spectrophotometry
  26. regression line
  27. manipulating equations
  28. line of best fit
  29. graphing by hand

Alignments

  1. Resource Type
  2. Audience Level
  3. Activity Length
  4. Inclusive Pedagogy for Life Science Education
Contents:
view all files

Description

Upon completion of this lab activity, the student will be able to:

  • Describe the components of an enzymatic reaction.
  • Communicate the function of an enzyme’s active site and its relationship to the substrate.
  • Describe the relationship between an enzyme’s structure and its function.
  • Explain the relationship between maltose produced and enzymatic (amylase) activity.
  • Analyze the effect of environmental factors on enzymatic activity.
  • Define absorbance and transmittance and utilize data obtained with a spectrophotometer.
  • Describe the relationship between absorbance and concentration.
  • Construct a standard curve using known amounts of maltose.
  • Utilize a standard curve to determine enzyme activity of the experimental amylase.

Adapted from Amylase Activity Lab, Montgomery College, Rockville, MD (doi:10.25334/VKGJ-VT46).

Cite this work

Researchers should cite this work as follows:

Close

Home

Search