Analysis of Amylase Activity
Author(s): Allison Carter Burlyn1, Christina Fieber1, Melanie Lenahan2
1. Horry-Georgetown Technical College 2. Raritan Valley Community College
1425 total view(s), 15973 download(s)
Summary:
This lab is an exploration of enzyme function and the effects of environmental conditions on the activity of the enzyme amylase. The lab utilizes analytical and graphing skills to assess enzyme activity.
Contents:
Enzyme Activity Instructor Guide.docx(DOCX | 3 MB)
Enzyme Activity Instructor Guide.pdf(PDF | 579 KB)- Enzyme Activity Post-Lab Assessment.docx(DOCX | 2 MB)
- Enzyme Activity Post-Lab Assessment.pdf(PDF | 123 KB)
- Enzyme Activity Pre-Lab Assessment.docx(DOCX | 2 MB)
- Enzyme Activity Pre-Lab Assessment.pdf(PDF | 54 KB)
- Enzyme Activity.docx(DOCX | 3 MB)
- Enzyme Activity.pdf(PDF | 410 KB)
- License terms
Description
Upon completion of this lab activity, the student will be able to:
- Describe the components of an enzymatic reaction.
- Communicate the function of an enzyme’s active site and its relationship to the substrate.
- Describe the relationship between an enzyme’s structure and its function.
- Explain the relationship between maltose produced and enzymatic (amylase) activity.
- Analyze the effect of environmental factors on enzymatic activity.
- Define absorbance and transmittance and utilize data obtained with a spectrophotometer.
- Describe the relationship between absorbance and concentration.
- Construct a standard curve using known amounts of maltose.
- Utilize a standard curve to determine enzyme activity of the experimental amylase.
Adapted from Amylase Activity Lab, Montgomery College, Rockville, MD (doi:10.25334/VKGJ-VT46).
Cite this work
Researchers should cite this work as follows:
- Burlyn, A. C., Fieber, C., Lenahan, M. (2020). Analysis of Amylase Activity. Catalytic Graphers, QUBES Educational Resources. doi:10.25334/CW0W-Y495