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    Long-read Sequencing Technology
    This 50 minute lecture on long-read sequencing technology covers the following items: 1) Review of RNA-Seq Short-read Sequencing, 2) Overview and benefits of Long-read Sequencing, 3) Oxford...

    Keywords: sequencingDNA sequenceRNA sequencingNanoporepacbiopacific biosciences

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    Microbiome Analysis with Oxford Nanopore 16S amplicon sequencing data
    The resource is a brief description of an analysis pipeline for microbiome analysis of Oxford Nanopore 16S amplicon sequence data. We tested the Kraken 2 and Krona pipeline from nanogalaxy with...

    Keywords: microbiome analysis toolNanopore

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    From Nano to Life: Interpreting graphs from Nanopore sequencing of bacterial genomes
    In this lesson, learners meet several scientists working on sustainable ways of recycling discarded electronics. Through an interactive H5P activity, participants watch videos and complete a series...

    Keywords: graph interpretationfmnNanoporeRepresentationBioGraphIrepresentation in STEMNanopore sequencing

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    REMNet: Introduction To Instructors 5.3.19
    Video on incorporating microbiome sequencing into biology education from the Research Experiences in Microbiomes Network

    Keywords: VideomicrobiomesREMNetmicrobiome sequencing

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    Advances in Genome Sequencing with Nathan Olson
    In this Lunch with a Scientist episode, we discuss Genome Sequencing and Bioinformatics with National Institute of Standards and Technology (NIST) biologist Nate Olson, Ph.D.

    Keywords: Bioinformatics and Genomics

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    Sanger Sequencing by Hand: Using Paper Clips to Demonstrate Chain Termination
    Sanger sequencing is commonly taught with a hands-on approach. Sanger sequencing involves chain termination by dideoxynucleotides, because they are missing the oxygen on the 3’ carbon atom,...

    Keywords: nucleotidessequencingDNAgel electrophoresisChain Termination

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    GEA Introductory lesson on RNA-Seq
    This RNA-Seq lesson uses the CyVerse VICE Platform and Jupyter notebooks to examine the expression of the leptin gene in samples from a mouse experimental design evaluating diet (high-fat vs....

    Keywords: data sciencebioinformaticsGenome BrowserRNAseqhigh throughput sequencingmRNAAlignmentNCBI Sequence Read Archive

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    A Fun Introductory Command Line Exercise: Next Generation Sequencing Quality Analysis with Emoji!
    This resource is a fun computer-based intro to command line programming. The activity takes FASTQ NGS data files and runs a fun program called FASTQE.

    Keywords: data sciencebioinformaticsnext generation sequencingquality controlFASTQgenomicsprogrammingNIBLSElinuxmicrobiomeIllumina sequencingcommand lineFASTQEFASTPNGS analysisterminalMacemojiPhred scoreTrimmingshort read

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    Intro to DNA Barcoding
    This is a classroom tested introductory lesson & activity to introduce DNA Barcoding and DNA Subway Blue Line. It is for novices and will fit into a 30-45 min lecture or lab class period.

    Keywords: phylogeneticsNIBLSEDNA BarcodingDNA sequenceBLASTmultiple sequence alignment

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    228

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    The Human Microbiome Biodiversity in Health and Disease
    The students will analyze the human gut and vaginal microbiomes in healthy and diseased states using diversity of bacteria as determined by 16SrRNA sequence.
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    Build-a-Genome Lab Manual for RFP Synthesis
    This lab manual describes a multi-week lab project for the design, assembly from oliognucleotides, cloning, and sequencing of the RFP gene. This resource was developed as part of NSF Award...
    877

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    505

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    REMNet Tutorial, R Part 5: Normalizing Microbiome Data in R 5.2.19
    Video on normalizing microbiome data from the Research Experiences in Microbiomes Network

    Keywords: geneticsstatisticsdata analysisgraphingRStudioVideoexperimental designR programming languagemicrobiomesstudent researchinterdisciplinary educationlimnologyeco-informaticsresearch in the classroomtidyverseWorking with datasetsREMNet

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    REMNet - A National Network for Integrating the Study of Microbiomes into Course-based Undergraduate Research Experiences (RCN-UBE Introduction)
    The mission of REMNet’s “Microbiomes for All!” is to serve as a centralized hub for information and resources on the integration of microbiome studies into the undergraduate curriculum. RemNet...

    Keywords: RCN-UBE Introduction

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    Microbiomes forAll: The Research Experiences in Microbiomes Network
    Poster on REMNet presented at the 2020 BIOME Institute: Cultivating Scientific Curiosity

    Keywords: undergraduate researchcourse-based undergraduate research experiencemicrobiomesmicrobiomeCUREREMNet

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    Tackling "Big Data" with Biology Undergrads: A Simple RNA-seq Data Analysis Tutorial Using Galaxy
    Analyzing high-throughput DNA sequence data is a fundamental skill in modern biology. However, real and perceived barriers such as massive file sizes, substantial computational requirements, and...

    Keywords: bioinformaticsDNA sequenceRNAseqgene expression

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    Teaching RNAseq at Undergraduate Institutions: A tutorial and R package from the Genome Consortium for Active Teaching
    This lesson plan was created to teach RNAseq analysis as a part of GCAT-SEEK network. It is provided here, both in finished form and with the modifiable source code, to allow flexible adaptation to...

    Keywords: statisticsR programming languagebioinformaticsNIBLSERNAseqgene expressionCourseSourcecomputer programming

    1955

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    521

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    Murder by HIV? Undergraduate edition
    Bioinformatics case study focusing on phylogenetic tree interpretation, published in the National Center for Case Study Teaching in Science

    Keywords: case studyResources @ NSF - NCCSTSbioinformaticsNIBLSEHIVnucleotide sequencingphylogenetic treeviral life cyclesmutation ratesreverse transcriptase sequences

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    Sequence Similarity: A Quick Introduction to Bioconductor
    Following the Sequence Similarity exercises by Kleinschmit, et al., this tutorial will guide students in using the Bioconductor suite of R packages to likewise compute sequence similarity.

    Keywords: bioinformaticssequencingNIBLSEmultiple sequence alignmentsequence similarityBLOSUM scoring matrixBioconductor

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    The Ames Test
    Introduction to the Ames Test, published as GSA Learning Resource

    Keywords: geneticsNIBLSEDNAames testassay

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    260

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    Yeti or not: Do they exist?
    Through this 4-part bioinformatics case study, students will be led through the forensic analysis of putative Yeti artifacts based on published findings.

    Keywords: case studybioinformaticsNIBLSEBLAST12S sequencingYeti

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