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CRISPR Gene Editing: Designing the gRNA and Donor Template

Author(s): Laurel Lorenz

Princeton

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Summary:
In this adaptation, students learn how CRISPR/Cas9 is used in bacterial immunity and gene editing. Students create both a gRNA target and a donor template to edit a gene. Mutations can be from the case study, Piwi Matter, or designed by the…

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In this adaptation, students learn how CRISPR/Cas9 is used in bacterial immunity and gene editing. Students create both a gRNA target and a donor template to edit a gene. Mutations can be from the case study, Piwi Matter, or designed by the instructor.

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

Version 1.0 - published on 15 Jul 2020 doi:10.25334/09G0-AS56 - cite this

Adapted from: Piwi Matters v 1.0

Keywords

  1. genetics
  2. education
  3. cell biology
  4. molecular biology
  5. mutation
  6. Stem cells
  7. structure-function
  8. molecular visualization and analysis
  9. 3D protein structure /function analysis
  10. CRISPR/Cas9
  11. crispr
  12. DNA mutation
  13. Piwi
  14. PDB
  15. Molecular Case Study
  16. Piwi Matters

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  1. Resource Type
  2. Audience Level
  3. Activity Length
  4. Inclusive Pedagogy for Life Science Education
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