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CRISPR Gene Editing: Designing the gRNA and Donor Template

Author(s): Laurel Lorenz

Princeton

2600 total view(s), 822 download(s)

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Summary:
In this adaptation, students learn how CRISPR/Cas9 is used in bacterial immunity and gene editing. Students create both a gRNA target and a donor template to edit a gene. Mutations can be from the case study, Piwi Matter, or designed by the…

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In this adaptation, students learn how CRISPR/Cas9 is used in bacterial immunity and gene editing. Students create both a gRNA target and a donor template to edit a gene. Mutations can be from the case study, Piwi Matter, or designed by the instructor.

Licensed under CC Attribution-ShareAlike 4.0 International according to these terms

Version 1.0 - published on 15 Jul 2020 doi:10.25334/09G0-AS56 - cite this

Adapted from: Piwi Matters v 1.0

Keywords

  1. genetics
  2. education
  3. cell biology
  4. molecular biology
  5. mutation
  6. Stem cells
  7. structure-function
  8. molecular visualization and analysis
  9. 3D protein structure /function analysis
  10. CRISPR/Cas9
  11. crispr
  12. DNA mutation
  13. Piwi
  14. PDB
  15. Molecular Case Study
  16. Piwi Matters

Alignments

  1. Resource Type
  2. Audience Level
  3. Activity Length
  4. Inclusive Pedagogy for Life Science Education
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