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CRISPR Gene Editing: Designing the gRNA and Donor Template

Author(s): Laurel Lorenz

Princeton

553 total view(s), 184 download(s)

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Summary:
In this adaptation, students learn how CRISPR/Cas9 is used in bacterial immunity and gene editing. Students create both a gRNA target and a donor template to edit a gene. Mutations can be from the case study, Piwi Matter, or designed by the…

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In this adaptation, students learn how CRISPR/Cas9 is used in bacterial immunity and gene editing. Students create both a gRNA target and a donor template to edit a gene. Mutations can be from the case study, Piwi Matter, or designed by the instructor.

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Total Views

553

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The above numbers reflect non-unique views of the page, which may include multiple views in the same day by the same user.

Total Downloads

184

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The above numbers reflect non-unique user access to the linked content, which includes but is not limited to primary access button clicks, bundle downloads, and supporting documents.

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