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    Long-read Sequencing Technology
    This 50 minute lecture on long-read sequencing technology covers the following items: 1) Review of RNA-Seq Short-read Sequencing, 2) Overview and benefits of Long-read Sequencing, 3) Oxford...

    Keywords: sequencingDNA sequenceRNA sequencingNanoporepacbiopacific biosciences

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    RNA-seq Analysis
    In this computer lab module, students learn how to process an RNA-seq data set to identify differentially expressed genes (DEGs). The samples for this data set were collected from yeast cells...

    Keywords: LabRNAsequencingUndergraduateNIBLSEincubatorsRNAseqInstructional SettingAudience LevelActivity LengthMore than 1 hourC4. Bioinformatics toolsC5. Data retrievalReviewed at NIBLSEdifferentially expressed genes

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    GEA Introductory lesson on RNA-Seq
    This RNA-Seq lesson uses the CyVerse VICE Platform and Jupyter notebooks to examine the expression of the leptin gene in samples from a mouse experimental design evaluating diet (high-fat vs....

    Keywords: data sciencebioinformaticsGenome BrowserRNAseqhigh throughput sequencingmRNAAlignmentNCBI Sequence Read Archive

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    Tackling "Big Data" with Biology Undergrads: A Simple RNA-seq Data Analysis Tutorial Using Galaxy
    Analyzing high-throughput DNA sequence data is a fundamental skill in modern biology. However, real and perceived barriers such as massive file sizes, substantial computational requirements, and...

    Keywords: bioinformaticsDNA sequenceRNAseqgene expression

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    Making bioinformatics tools classroom-friendly
    Poster on using Cyverse resources to make classroom that make using bioinformatics in the classroom a more manageable experience presented at the 2020 BIOME Institute: Cultivating Scientific Curiosity

    Keywords: bioinformaticsJupyterJupyter notebookBLASTCyVerseRNA sequencing

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    REMNet - A National Network for Integrating the Study of Microbiomes into Course-based Undergraduate Research Experiences (RCN-UBE Introduction)
    The mission of REMNet’s “Microbiomes for All!” is to serve as a centralized hub for information and resources on the integration of microbiome studies into the undergraduate curriculum. RemNet...

    Keywords: RCN-UBE Introduction

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    Dynamic Programming
    In bioinformatics, dynamic programming is mainly used to align nucleotide and amino acid sequences, and to predict RNA and protein folding from primary sequence data.

    Keywords: RNAamino acid sequenceSequence alignmentdynamic programmingprotein folding

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    Aging and Circular RNAs
    A science FIT seminar presented to incoming freshmen at UNR

    Keywords: videosbioinformaticsRNAaginggenomicsyoutubecircular rna

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    Single Cell Insights Into Cancer Transcriptomes: A Five-Part Single-Cell RNAseq Case Study Lesson
    There is a growing need for integration of “Big Data” into undergraduate biology curricula. Transcriptomics is one venue to examine biology from an informatics perspective. RNA sequencing has...

    Keywords: bioinformaticscancertranscriptomicshigh-throughputData wranglingSingle cell RNA sequencingq

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    Module 4: Removal of Introns from pre-mRNA by Splicing
    In this module, students will learn to identify splice donor and acceptor sites that are best supported by RNA-Seq data, and use the canonical splice donor and splice acceptor sequences to identify...

    Keywords: geneticsdata visualizationmolecular biologygenomicsexonintronsplicingSimilarity searchesRNA Maturation

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    REMNet: Introduction To Instructors 5.3.19
    Video on incorporating microbiome sequencing into biology education from the Research Experiences in Microbiomes Network

    Keywords: VideomicrobiomesREMNetmicrobiome sequencing

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    Using Raw Sequence Data to Analyze Tumor Suppressor Mutations
    Learning to identify DNA sequence variants from raw sequencing data (electropherograms) is a basic molecular biology skill with applications to basic research and clinical practice. Yet learning to...

    Keywords: bioinformaticsmutationDNAgenomic visualizationparts of a geneclinical consequencesraw data

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    Genomics Lessons with GEA - RNA-Seq CURES
    Our BIOME Group worked towards developing a set of learning goals for faculty who plan to launch a CURE based on RNA-Seq data. We also developed a workflow for analysis of either prokaryotic or...
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    Building creatures with the central dogma of biology
    Activity that demonstrates how nucleotide changes can affect phenotypes

    Keywords: amino acidsnucleotidesRNADNAgenotypephenotypecentral dogma

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    Advances in Genome Sequencing with Nathan Olson
    In this Lunch with a Scientist episode, we discuss Genome Sequencing and Bioinformatics with National Institute of Standards and Technology (NIST) biologist Nate Olson, Ph.D.

    Keywords: Bioinformatics and Genomics

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    From Nano to Life: Interpreting graphs from Nanopore sequencing of bacterial genomes
    In this lesson, learners meet several scientists working on sustainable ways of recycling discarded electronics. Through an interactive H5P activity, participants watch videos and complete a series...

    Keywords: graph interpretationfmnNanoporeRepresentationBioGraphIrepresentation in STEMNanopore sequencing

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    Module 3: Transcription, Part II: What Happens to the Initial Transcript Made by RNA pol II?
    This module teaches about the three key steps that are involved in converting the pre-mRNA into a mature mRNA: 1) The addition of a 5’ cap, 2) The addition of a 3’ poly(A) tail, 3) The removal of...

    Keywords: geneticsmolecular biologyGenome BrowsergenomicssplicingmRNAcDNA5' Cap3' Poly(A) TailRNA Maturation

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    Teaching RNAseq at Undergraduate Institutions: A tutorial and R package from the Genome Consortium for Active Teaching
    Next-generation sequencing is radically changing the study of biology, but there are currently few resources aimed at teaching the required laboratory and data-analysis skills to undergraduate...

    Keywords: statisticsbioinformaticsRNAseqgene expressioncomputer programming

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    Using CRISPR-Cas9 to teach the fundamentals of molecular biology and experimental design
    One of the challenges of inquiry-based research experiences is balancing the instruction on skills and techniques with data generation and analysis. This is especially true in technique heavy...

    Keywords: experimental designcrisprmolecular technique

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    Microbiome Analysis with Oxford Nanopore 16S amplicon sequencing data
    The resource is a brief description of an analysis pipeline for microbiome analysis of Oxford Nanopore 16S amplicon sequence data. We tested the Kraken 2 and Krona pipeline from nanogalaxy with...

    Keywords: microbiome analysis toolNanopore

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