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Build-a-Genome Lab Manual for RFP Synthesis
28 Jul 2020 | Teaching Materials | Contributor(s):
By Lisa Scheifele
Loyola University Maryland
This lab manual describes a multi-week lab project for the design, assembly from oliognucleotides, cloning, and sequencing of the RFP gene.
Anti-bias Education Puppet Plays
By Julia Robinson
Cecilia Berg Center for Environmental Education; Hefner Museum of Natural History; Miami University
Nature provides science content for teacher preparation certification programs and the backdrop for puppet plays used as a springboard for teaching the NAEYC's four goals of Anti-bias...
Yeast Scramble Lab Manual
By Mark Jones, Lisa Scheifele1
This laboratory manual includes protocols for SCRaMbling of yeast cells as well as phenotypic characterization. Appropriate student exercises are also included.
Understanding Eukaryotic Genes modules: Glossary of terms
25 Jul 2020 | Teaching Materials | Contributor(s):
By Meg Laakso1, Carina Howell2, Cathy Silver Key3, Leocadia Paliulis4, Maria S Santisteban5, Chiyedza Small6, Joyce Stamm7, Elena Gracheva8
1. Eastern University 2. Lock Haven University 3. North Carolina Central University 4. Bucknell University 5. UNC at Pembroke 6. Medgar Evers College CUNY 7. University of Evansville 8. Washington University in St. Louis
This glossary defines the key terms that are used in the Understanding Eukaryotic Genes modules.
Module 6: Alternative Splicing
By Leocadia Paliulis1, Anne Rosenwald2
1. Bucknell University 2. Georgetown University
This module explores how multiple different mRNAs and polypeptides can be encoded by the same gene. After completing this module students will be able to explain how alternative splicing of a gene...
Module 5: The Need for an Open Reading Frame
By Carina Howell1, Leocadia Paliulis2, Anne Rosenwald3
1. Lock Haven University 2. Bucknell University 3. Georgetown University
In this module, students will learn to identify the open reading frames for a given gene, and define the phases of the splice donor and acceptor sites.
Module 4: Removal of Introns from pre-mRNA by Splicing
By Meg Laakso1, Anne Rosenwald2
1. Eastern University 2. Georgetown University
In this module, students will learn to identify splice donor and acceptor sites that are best supported by RNA-Seq data, and use the canonical splice donor and splice acceptor sequences to identify...
Module 3: Transcription, Part II: What Happens to the Initial Transcript Made by RNA pol II?
By Cathy Silver Key1, Chiyedza Small2, Laura Reed3
1. North Carolina Central University 2. Medgar Evers College CUNY 3. The University of Alabama
This module teaches about the three key steps that are involved in converting the pre-mRNA into a mature mRNA: 1) The addition of a 5’ cap, 2) The addition of a 3’ poly(A) tail, 3) The removal of...
Module 2: Transcription Part I: From DNA Sequence to Transcription Unit
By Maria Santisteban1, Alexa Sawa2
1. University of North Carolina - Pembroke 2. College of the Desert
This module illustrates how a primary transcript (pre-mRNA) is synthesized using a DNA molecule as the template. Students will learn about the importance of the 5' and 3' regions of the gene for...
Module 1: Introduction to the Genome Browser: What is a Gene?
By Joyce Stamm1, Jennifer Kennell2, Wilson Leung3, Leocadia Paliulis4, Srebrenka Robic5, Anne Rosenwald6
1. University of Evansville 2. Vassar College 3. Washington University in St. Louis 4. Bucknell University 5. Agnes Scott College 6. Georgetown University
This lesson introduces the University of California Santa Cruz genome browser to students, walking them through some of the key features so that it can be used for analysis of gene structure.
GEA Introductory lesson on RNA-Seq
By Jason Williams1, Judy Brusslan2, Ray Enke3, Matthew Escobar4, Vince Buonaccorsi5
1. Cold Spring Harbor Laboratory 2. California State University Long Beach 3. James Madison University 4. California State University San Marcos 5. Juniata College
This RNA-Seq lesson uses the CyVerse VICE Platform and Jupyter notebooks to examine the expression of the leptin gene in samples from a mouse experimental design evaluating diet (high-fat vs....
GEA Introductory lesson on BLAST
By Justin DiAngelo1, Alexis Nagengast2, Wilson Leung3, Douglas L Chalker3
1. Penn State Berks 2. Widener University 3. Washington University in St. Louis
The "Introduction to BLAST using Human Leptin" exercise aims to introduce students to the use of the Basic Local Alignment Search Tool (BLAST) to identify related sequences and compare similarity...
NEON Data in the Classroom: Quantifying Spatial Patterns
23 Jul 2020 | Teaching Materials | Contributor(s):
By Kusum Naithani
University of Arkansas, Fayetteville, AR 72701
Students build on fundamental concepts of spatial patterns and combine this knowledge with the open-data from the National Ecological Observatory Network to quantify spatial autocorrelation and...
Creating Phylogenetic Trees from DNA Sequences
22 Jul 2020 | Teaching Materials | Contributor(s):
By HHMI BioInteractive
This interactive module shows how DNA sequences can be used to infer evolutionary relationships among organisms and represent them as phylogenetic trees.
Pivoting Intro from Inquiry to Online
21 Jul 2020 | Teaching Materials | Contributor(s):
By J. Phil Gibson
University of Oklahoma
This poster will present how inquiry-based learning experiences that were designed for face-to-face labs can be modified in a rapid pivot to online teaching.
Comparing Primary and Secondary Forest in a Preserve in Plano Texas: A Forest Ecology Course-based Undergraduate Research Experience (CURE) for Non-Majors and Lower-Level Majors
By Tamara Basham
Collin County Commuity College District
An activity that can be conducted as an online or field exercise in which students develop and test their hypotheses about how forest stand characteristics differ between two forest types.
Climate-Induced Shifts in California Butterflies
17 Jul 2020 | Teaching Materials | Contributor(s):
By Jessica Coyle
A module in which students generate and test hypotheses about butterfly species’ response to climate change in the Central Valley and Sierra Nevada Mountains of northern California using publicly...
Cleaning Biodiversity Data: A Botanical Example Using Excel or RStudio
16 Jul 2020 | Teaching Materials | Contributor(s):
By Michelle Gaynor
University of Florida
Access and clean an open source herbarium dataset using Excel or RStudio.
CRISPR Gene Editing: Designing the gRNA and Donor Template
15 Jul 2020 | Teaching Materials | Contributor(s):
By Laurel Lorenz
In this adaptation, students learn how CRISPR/Cas9 is used in bacterial immunity and gene editing. Students create both a gRNA target and a donor template to edit a gene. Mutations can be from the...
A New Phenology-focused CURE using Herbarium Specimen Data
14 Jul 2020 | Teaching Materials | Contributor(s):
By Katie Pearson1, Natalie Love2, Tadeo Parada2, Jenn Yost
1. Cal Poly State University 2. UC Santa Barbara
A short description of the undergraduate research course developed by the California Phenology Network.